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@ARTICLE{Surowka:311052,
      author       = {Surowka, A. D. and Töpperwien, M. and Bernhardt, M. and
                      Nicolas, J. D. and Osterhoff, M. and Salditt, T. and Adamek,
                      D. and Szczerbowska-Boruchowska, Magdalena},
      title        = {{C}ombined in-situ imaging of structural organization and
                      elemental composition of substantia nigra neurons in the
                      elderly},
      journal      = {Talanta},
      volume       = {161},
      issn         = {0039-9140},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {PUBDB-2016-04601},
      pages        = {368 - 376},
      year         = {2016},
      note         = {(c) Elsevier B.V.},
      abstract     = {Human dopaminergic system in general, and substantia nigra
                      (SN) neurons, in particular, are implicated in the
                      pathologies underlying the human brain aging. The interplay
                      between aberrations in the structural organization and
                      elemental composition of SN neuron bodies has recently
                      gained in importance as selected metals: Fe, Cu, Zn, Ca were
                      found to trigger oxidative-stress-mediated aberration in
                      their molecular assembly due to concomitant protein
                      (alpha-synuclein, tau-protein) aggregation, gliosis and
                      finally oxidative stress. In the present study, we
                      demonstrate an integrated approach to the analysis of the
                      structural organization, assembly, and metals’
                      accumulation in two distinct areas of SN: in the
                      neuro-melanin neurons and neuropil. By using the highly
                      brilliant source of PETRA III and the
                      Kirkpatrick-Baeznano-focus, large area histological brain
                      slices are scanned at the sub-neuronal resolution, taking
                      advantage of continuous motor movement and reduced
                      acquisition time. Elemental analysis with synchrotron
                      radiation based X-ray Fluorescence (SRXRF) is combined with
                      X-ray Phase Contrast Imaging (XPCI) to correct for inherent
                      aberrations in the samples’ density and thickness, often
                      referred to as the mass thickness effect. Based on the raw
                      SRXRF spectra, we observed the accumulation of P, S, Cl, K,
                      Ca, Fe, Cu and Zn predominantly in the SN neurons. However,
                      upon the mass thickness correction, the distributions of Cl
                      became significantly more uniform. Simultaneously with the
                      fluorescence signal, the Small Angle X-ray Scattering (SAXS)
                      is recorded by a pixel detector positioned in the far-field,
                      enabling fast online computation of the dark field and
                      differential phase contrast (DPC). The data has demonstrated
                      the SN neurons and neuropil produces excellent contrast
                      which is due to their different mass density and scattering
                      strength, indicative of differences in local structure and
                      assembly therein. In all, the results show that combined
                      SRXRF-XPCI-SAXS experiments can robustly serve as a unique
                      tool for understanding the interplay between the chemical
                      composition and structural organization that may drive the
                      biochemical age-related processes occurring in the human
                      dopaminergic system.},
      cin          = {DOOR},
      ddc          = {540},
      cid          = {I:(DE-H253)HAS-User-20120731},
      pnm          = {6G3 - PETRA III (POF3-622) / FS-Proposal: I-20140024 EC
                      (I-20140024-EC) / CALIPSO - Coordinated Access to
                      Lightsources to Promote Standards and Optimization (312284)},
      pid          = {G:(DE-HGF)POF3-6G3 / G:(DE-H253)I-20140024-EC /
                      G:(EU-Grant)312284},
      experiment   = {EXP:(DE-H253)P-P10-20150101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000386989500046},
      pubmed       = {pmid:27769419},
      doi          = {10.1016/j.talanta.2016.08.023},
      url          = {https://bib-pubdb1.desy.de/record/311052},
}