TY - JOUR
AU - Coughlan, H. D.
AU - Darmanin, C.
AU - Phillips, N. W.
AU - Hofmann, F.
AU - Clark, Jesse
AU - Harder, R. J.
AU - Vine, D. J.
AU - Abbey, B.
TI - Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
JO - Structural dynamics
VL - 2
IS - 4
SN - 2329-7778
CY - Melville, NY
PB - AIP Publishing LLC
M1 - PUBDB-2016-00562
SP - 041704 -
PY - 2015
AB - For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractiveimaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.
LB - PUB:(DE-HGF)16
UR - <Go to ISI:>//WOS:000360649200006
C6 - pmid:26798804
DO - DOI:10.1063/1.4919641
UR - https://bib-pubdb1.desy.de/record/293473
ER -