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@ARTICLE{Tallant:293458,
author = {Tallant, Cynthia and Valentini, Erica and Fedorov, Oleg and
Overvoorde, Lois and Ferguson, Fleur M. and
Filippakopoulos, Panagis and Svergun, Dmitri I. and Knapp,
Stefan and Ciulli, Alessio},
title = {{M}olecular {B}asis of {H}istone {T}ail {R}ecognition by
{H}uman {TIP}5 {PHD} {F}inger and {B}romodomain of the
{C}hromatin {R}emodeling {C}omplex {N}o{RC}},
journal = {Structure},
volume = {23},
number = {1},
issn = {0969-2126},
address = {London [u.a.]},
publisher = {Elsevier Science},
reportid = {PUBDB-2016-00547},
pages = {80 - 92},
year = {2015},
abstract = {Binding of the chromatin remodeling complex NoRCto RNA
complementary to the rDNA promoter medi-ates transcriptional
repression. TIP5, the largest sub-unit of NoRC, is involved
in recruitment to rDNA byinteractions with promoter-bound
TTF-I, pRNA, andacetylation of H4K16. TIP5 domains that
recognizeposttranslational modifications on histones
areessential for recruitment of NoRC to chromatin, buthow
these reader modules recognize site-specifichistone tails
has remained elusive. Here, we reportcrystal structures of
PHD zinc finger and bromodo-mains from human TIP5 and BAZ2B
in free form andbound to H3 and/or H4 histones. PHD finger
functionsas an independent structural module in
recognizingunmodified H3 histone tails, and the
bromodomainprefers H3 and H4 acetylation marks followed by
akey basic residue, KacXXR. Further low-resolutionanalyses
of PHD-bromodomain modules providemolecular insights into
their trans histone tail recogni-tion, required for
nucleosome recruitment and tran-scriptional repression of
the NoRC complex.},
cin = {EMBL / EMBL-User},
ddc = {570},
cid = {I:(DE-H253)EMBL-20120731 / I:(DE-H253)EMBL-User-20120814},
pnm = {899 - ohne Topic (POF3-899) / BIOSTRUCT-X - Transnational
access and enhancement of integrated Biological Structure
determination at synchrotron X-ray radiation facilities
(283570) / IDPBYNMR - High resolution tools to understand
the functional role of protein intrinsic disorder (264257) /
6G3 - PETRA III (POF3-622)},
pid = {G:(DE-HGF)POF3-899 / G:(EU-Grant)283570 /
G:(EU-Grant)264257 / G:(DE-HGF)POF3-6G3},
experiment = {EXP:(DE-H253)DORISIII(machine)-20150101 /
EXP:(DE-H253)P-P12-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000347469500013},
pubmed = {pmid:25533489},
doi = {10.1016/j.str.2014.10.017},
url = {https://bib-pubdb1.desy.de/record/293458},
}