TY  - JOUR
AU  - Tallant, Cynthia
AU  - Valentini, Erica
AU  - Fedorov, Oleg
AU  - Overvoorde, Lois
AU  - Ferguson, Fleur M.
AU  - Filippakopoulos, Panagis
AU  - Svergun, Dmitri I.
AU  - Knapp, Stefan
AU  - Ciulli, Alessio
TI  - Molecular Basis of Histone Tail Recognition by Human TIP5 PHD Finger and Bromodomain of the Chromatin Remodeling Complex NoRC
JO  - Structure
VL  - 23
IS  - 1
SN  - 0969-2126
CY  - London [u.a.]
PB  - Elsevier Science
M1  - PUBDB-2016-00547
SP  - 80 - 92
PY  - 2015
AB  - Binding of the chromatin remodeling complex NoRCto RNA complementary to the rDNA promoter medi-ates transcriptional repression. TIP5, the largest sub-unit of NoRC, is involved in recruitment to rDNA byinteractions with promoter-bound TTF-I, pRNA, andacetylation of H4K16. TIP5 domains that recognizeposttranslational modifications on histones areessential for recruitment of NoRC to chromatin, buthow these reader modules recognize site-specifichistone tails has remained elusive. Here, we reportcrystal structures of PHD zinc finger and bromodo-mains from human TIP5 and BAZ2B in free form andbound to H3 and/or H4 histones. PHD finger functionsas an independent structural module in recognizingunmodified H3 histone tails, and the bromodomainprefers H3 and H4 acetylation marks followed by akey basic residue, KacXXR. Further low-resolutionanalyses of PHD-bromodomain modules providemolecular insights into their trans histone tail recogni-tion, required for nucleosome recruitment and tran-scriptional repression of the NoRC complex.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000347469500013
C6  - pmid:25533489
DO  - DOI:10.1016/j.str.2014.10.017
UR  - https://bib-pubdb1.desy.de/record/293458
ER  -