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@ARTICLE{Fodor:293439,
author = {Fodor, Krisztián and Wolf, Janina and Reglinski, Katharina
and Passon, Daniel M. and Lou, Ye and Schliebs, Wolfgang and
Erdmann, Ralf and Wilmanns, Matthias},
title = {{L}igand-{I}nduced {C}ompaction of the {PEX}5
{R}eceptor-{B}inding {C}avity {I}mpacts {P}rotein {I}mport
{E}fficiency into {P}eroxisomes},
journal = {Traffic},
volume = {16},
number = {1},
issn = {1398-9219},
address = {Oxford},
publisher = {Wiley-Blackwell},
reportid = {PUBDB-2016-00528},
pages = {85 - 98},
year = {2015},
note = {(c) John Wiley $\&$ Sons A/S. Published by John Wiley $\&$
Sons Ltd. Post referee full text in progress.},
abstract = {Peroxisomes entirely rely on the import of their proteome
across the peroxisomal membrane. Recognition efficiencies of
peroxisomal proteins vary by more than 1000-fold, but the
molecular rationale behind their subsequent differential
import and sorting has remained enigmatic. Using the protein
cargo alanine-glyoxylate aminotransferase as a model, an
unexpected increase from 34 to $80\%$ in peroxisomal import
efficiency of a single-residue mutant has been discovered.
By high-resolution structural analysis, we found that it is
the recognition receptor PEX5 that adapts its conformation
for high-affinity binding rather than the cargo protein
signal motif as previously thought. During receptor
recognition, the binding cavity of the receptor shrinks to
one third of its original volume. This process is impeded in
the wild-type protein cargo because of a bulky side chain
within the recognition motif, which blocks contraction of
the PEX5 binding cavity. Our data provide a new insight into
direct protein import efficiency by removal rather than by
addition of an apparent specific sequence signature that is
generally applicable to peroxisomal matrix proteins and to
other receptor recognition processes.},
cin = {EMBL},
ddc = {570},
cid = {I:(DE-H253)EMBL-20120731},
pnm = {899 - ohne Topic (POF3-899)},
pid = {G:(DE-HGF)POF3-899},
experiment = {EXP:(DE-H253)DORISIII(machine)-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000346733300006},
pubmed = {pmid:25369882},
doi = {10.1111/tra.12238},
url = {https://bib-pubdb1.desy.de/record/293439},
}