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@PHDTHESIS{Ren:221176,
      author       = {Ren, Ling},
      title        = {{U}ltrafast {D}esorption by {I}mpulsive {V}ibrational
                      {E}xcitation ({DIVE}): {A}pplications in {L}aser {S}urgery,
                      {M}ass {S}pectrometry and {T}owards {U}ltimate {L}imits in
                      {B}iodiagnosis},
      issn         = {1435-8085},
      school       = {Universität Hamburg},
      type         = {Dr.},
      publisher    = {Verlag Deutsches Elektronen-Synchrotron},
      reportid     = {PUBDB-2015-02552, DESY-THESIS-2015-023},
      series       = {DESY-THESIS},
      pages        = {109},
      year         = {2015},
      note         = {Universität Hamburg, Diss., 2015},
      abstract     = {The prospects for minimally invasive surgery, spatial
                      imaging with mass spectrometry and rapid high throughput
                      biodiagnosis require new means of tissue incision and
                      biomolecule extraction with conserved molecular structure.
                      Towards this aim, a laser ablation process is utilized in
                      this dissertation, which is capable of performing precise
                      tissue incision with minimal collateral damage and
                      extracting intact biological entities with conserved
                      biological functions. The method is based on the recently
                      developed Picosecond Infrared Laser (PIRL) designed to
                      excite selectively the water vibrational modes under the
                      condition of ultrafast Desorption by Impulsive Vibrational
                      Excitation (DIVE). The basic concept is that the selectively
                      excited water molecules act as propellant to ablate whole
                      biological complexes into the plume, faster than any thermal
                      deleterious effect or fragmentation that would mask
                      molecular identities.The PIRL ablation under DIVE condition
                      is applied for the first time to six types of ocular
                      tissues, rendering precise and minimally invasive incisions
                      in a well-controlled and reproducible way. An eminent
                      demonstration is the contact-free and applanation-free
                      corneal trephination with the PIRL. Mass spectrometry and
                      other analytical techniques show that great abundance of
                      proteins with various molecular weights are extracted from
                      the tissue by the PIRL ablation, and that fragmentation or
                      other chemical alternation does not occur to the proteins in
                      the ablation plume. With various microscope imaging and
                      biochemical analysis methods, nano-scale single protein
                      molecules, viruses and cells in the ablation plume are found
                      to be morphologically and functionally identical to their
                      corresponding controls. The PIRL ablation provides a new
                      means to push the frontiers of laser surgery in
                      ophthalmology and can be applied to resolve chemical
                      activities in situ and in vivo. The most important finding
                      is the conserved nature of the extracted biological
                      entities, rendering compositional reconstruction possible at
                      the spatial limit of the laser focus, which has conspicuous
                      potential in advancing rapid high throughput biodiagnosis.},
      cin          = {MPSD},
      cid          = {I:(DE-H253)MPSD-20120731},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
      pid          = {G:(DE-HGF)POF3-6215},
      experiment   = {EXP:(DE-H253)CFEL-Exp-20150101},
      typ          = {PUB:(DE-HGF)29 / PUB:(DE-HGF)11},
      doi          = {10.3204/DESY-THESIS-2015-023},
      url          = {https://bib-pubdb1.desy.de/record/221176},
}