000221176 001__ 221176
000221176 005__ 20211110131225.0
000221176 0247_ $$2datacite_doi$$a10.3204/DESY-THESIS-2015-023
000221176 037__ $$aPUBDB-2015-02552
000221176 041__ $$aEnglish
000221176 0881_ $$aDESY-THESIS-2015-023
000221176 088__ $$2DESY$$aDESY-THESIS-2015-023
000221176 1001_ $$0P:(DE-H253)PIP1015288$$aRen, Ling$$b0$$eCorresponding author$$gfemale
000221176 245__ $$aUltrafast Desorption by Impulsive Vibrational Excitation (DIVE): Applications in Laser Surgery, Mass Spectrometry and Towards Ultimate Limits in Biodiagnosis$$f2011-11-01 - 2015-06-29
000221176 260__ $$bVerlag Deutsches Elektronen-Synchrotron$$c2015
000221176 300__ $$a109
000221176 3367_ $$2DataCite$$aOutput Types/Dissertation
000221176 3367_ $$2ORCID$$aDISSERTATION
000221176 3367_ $$2BibTeX$$aPHDTHESIS
000221176 3367_ $$02$$2EndNote$$aThesis
000221176 3367_ $$0PUB:(DE-HGF)29$$2PUB:(DE-HGF)$$aReport$$mreport
000221176 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis$$bphd$$mphd$$s1454405311_21963
000221176 3367_ $$2DRIVER$$adoctoralThesis
000221176 4900_ $$0PERI:(DE-600)1437131-5$$aDESY-THESIS$$x1435-8085
000221176 502__ $$aUniversität Hamburg, Diss., 2015$$bDr.$$cUniversität Hamburg$$d2015
000221176 520__ $$aThe prospects for minimally invasive surgery, spatial imaging with mass spectrometry and rapid high throughput biodiagnosis require new means of tissue incision and biomolecule extraction with conserved molecular structure. Towards this aim, a laser ablation process is utilized in this dissertation, which is capable of performing precise tissue incision with minimal collateral damage and extracting intact biological entities with conserved biological functions. The method is based on the recently developed Picosecond Infrared Laser (PIRL) designed to excite selectively the water vibrational modes under the condition of ultrafast Desorption by Impulsive Vibrational Excitation (DIVE). The basic concept is that the selectively excited water molecules act as propellant to ablate whole biological complexes into the plume, faster than any thermal deleterious effect or fragmentation that would mask molecular identities.The PIRL ablation under DIVE condition is applied for the first time to six types of ocular tissues, rendering precise and minimally invasive incisions in a well-controlled and reproducible way. An eminent demonstration is the contact-free and applanation-free corneal trephination with the PIRL. Mass spectrometry and other analytical techniques show that great abundance of proteins with various molecular weights are extracted from the tissue by the PIRL ablation, and that fragmentation or other chemical alternation does not occur to the proteins in the ablation plume. With various microscope imaging and biochemical analysis methods, nano-scale single protein molecules, viruses and cells in the ablation plume are found to be morphologically and functionally identical to their corresponding controls. The PIRL ablation provides a new means to push the frontiers of laser surgery in ophthalmology and can be applied to resolve chemical activities in situ and in vivo. The most important finding is the conserved nature of the extracted biological entities, rendering compositional reconstruction possible at the spatial limit of the laser focus, which has conspicuous potential in advancing rapid high throughput biodiagnosis.
000221176 536__ $$0G:(DE-HGF)POF3-6215$$a6215 - Soft Matter, Health and Life Sciences (POF3-621)$$cPOF3-621$$fPOF III$$x0
000221176 650_7 $$xDiss.
000221176 693__ $$0EXP:(DE-H253)CFEL-Exp-20150101$$5EXP:(DE-H253)CFEL-Exp-20150101$$eExperiments at CFEL$$x0
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/Dissertation_Ling_REN.pdf$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/desy-thesis-15-023.title.pdf$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/Dissertation_Ling_REN.gif?subformat=icon$$xicon$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/Dissertation_Ling_REN.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/Dissertation_Ling_REN.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/Dissertation_Ling_REN.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/Dissertation_Ling_REN.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/desy-thesis-15-023.title.gif?subformat=icon$$xicon$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/desy-thesis-15-023.title.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/desy-thesis-15-023.title.jpg?subformat=icon-700$$xicon-700$$yOpenAccess
000221176 8564_ $$uhttps://bib-pubdb1.desy.de/record/221176/files/desy-thesis-15-023.title.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000221176 909CO $$ooai:bib-pubdb1.desy.de:221176$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000221176 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1015288$$aExternes Institut$$b0$$k>Extern
000221176 9131_ $$0G:(DE-HGF)POF3-621$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6215$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vIn-house research on the structure, dynamics and function of matter$$x0
000221176 9141_ $$y2015
000221176 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000221176 920__ $$lno
000221176 9201_ $$0I:(DE-H253)MPSD-20120731$$kMPSD$$lForschungsgruppe für strukturelle Dynamik$$x0
000221176 980__ $$aphd
000221176 980__ $$aVDB
000221176 980__ $$aUNRESTRICTED
000221176 980__ $$areport
000221176 980__ $$aI:(DE-H253)MPSD-20120731
000221176 9801_ $$aFullTexts