000220265 001__ 220265
000220265 005__ 20250717105523.0
000220265 0247_ $$2doi$$a10.1063/1.4919398
000220265 0247_ $$2WOS$$aWOS:000360649200005
000220265 0247_ $$2pmid$$apmid:26798803
000220265 0247_ $$2altmetric$$aaltmetric:3953412
000220265 0247_ $$2openalex$$aopenalex:W1991313455
000220265 037__ $$aPUBDB-2015-02182
000220265 082__ $$a530
000220265 1001_ $$0P:(DE-H253)PIP1013871$$aGalli, Lorenzo$$b0
000220265 245__ $$aElectronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse
000220265 260__ $$aMelville, NY$$bAIP Publishing LLC$$c2015
000220265 3367_ $$2DRIVER$$aarticle
000220265 3367_ $$2DataCite$$aOutput Types/Journal article
000220265 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1480921499_25134
000220265 3367_ $$2BibTeX$$aARTICLE
000220265 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000220265 3367_ $$00$$2EndNote$$aJournal Article
000220265 500__ $$aOA
000220265 520__ $$aCurrent hard X-ray free-electron laser (XFEL) sources can deliver doses to biological macromolecules well exceeding 1 GGy, in timescales of a few tens of femtoseconds. During the pulse, photoionization can reach the point of saturation in which certain atomic species in the sample lose most of their electrons. This electronic radiation damage causes the atomic scattering factors to change, affecting, in particular, the heavy atoms, due to their higher photoabsorption cross sections. Here, it is shown that experimental serial femtosecond crystallography data collected with an extremely bright XFEL source exhibit a reduction of the effective scattering power of the sulfur atoms in a native protein. Quantitative methods are developed to retrieve information on the effective ionization of the damaged atomic species from experimental data, and the implications of utilizing new phasing methods which can take advantage of this localized radiation damage are discussed.
000220265 536__ $$0G:(DE-HGF)POF3-6211$$a6211 - Extreme States of Matter: From Cold Ions to Hot Plasmas (POF3-621)$$cPOF3-621$$fPOF III$$x0
000220265 536__ $$0G:(DE-H253)BMBF-05K13GU7$$a05K13GU7 - Test eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05K13GU7)$$cBMBF-05K13GU7$$x1
000220265 536__ $$0G:(DE-HGF)2015_IFV-VH-GS-500$$aVH-GS-500 - PIER Helmholtz Graduate School (2015_IFV-VH-GS-500)$$c2015_IFV-VH-GS-500$$x2
000220265 588__ $$aDataset connected to CrossRef
000220265 693__ $$0EXP:(DE-MLZ)NOSPEC-20140101$$5EXP:(DE-MLZ)NOSPEC-20140101$$eNo specific instrument$$x0
000220265 7001_ $$0P:(DE-H253)PIP1012452$$aSon, S.-K.$$b1
000220265 7001_ $$aKlinge, M.$$b2
000220265 7001_ $$0P:(DE-H253)PIP1006443$$aBajt, S.$$b3$$udesy
000220265 7001_ $$0P:(DE-H253)PIP1008245$$aBarty, A.$$b4$$udesy
000220265 7001_ $$0P:(DE-H253)PIP1016993$$aBean, Richard$$b5
000220265 7001_ $$aBetzel, C.$$b6
000220265 7001_ $$0P:(DE-H253)PIP1016189$$aBeyerlein, Kenneth$$b7$$udesy
000220265 7001_ $$0P:(DE-H253)PIP1011740$$aCaleman, C.$$b8$$udesy
000220265 7001_ $$aDoak, R. B.$$b9
000220265 7001_ $$aDuszenko, M.$$b10
000220265 7001_ $$0P:(DE-H253)PIP1011659$$aFleckenstein, H.$$b11$$udesy
000220265 7001_ $$0P:(DE-H253)PIP1017962$$aGati, C.$$b12
000220265 7001_ $$aHunt, B.$$b13
000220265 7001_ $$aKirian, R. A.$$b14
000220265 7001_ $$aLiang, M.$$b15
000220265 7001_ $$aNanao, M. H.$$b16
000220265 7001_ $$aNass, K.$$b17
000220265 7001_ $$0P:(DE-H253)PIP1012087$$aOberthür, D.$$b18$$udesy
000220265 7001_ $$aRedecke, L.$$b19
000220265 7001_ $$aShoeman, R.$$b20
000220265 7001_ $$0P:(DE-H253)PIP1011192$$aStellato, F.$$b21$$udesy
000220265 7001_ $$0P:(DE-H253)PIP1016300$$aYoon, C. H.$$b22$$udesy
000220265 7001_ $$0P:(DE-H253)PIP1010779$$aWhite, T. A.$$b23$$udesy
000220265 7001_ $$0P:(DE-H253)PIP1006155$$aYefanov, O.$$b24$$udesy
000220265 7001_ $$aSpence, J.$$b25
000220265 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, H. N.$$b26$$eCorresponding author
000220265 773__ $$0PERI:(DE-600)2758684-4$$a10.1063/1.4919398$$gVol. 2, no. 4, p. 041703 -$$n4$$p041703$$tStructural dynamics$$v2$$x2329-7778$$y2015
000220265 8564_ $$yOpenAccess
000220265 8564_ $$uhttps://bib-pubdb1.desy.de/record/220265/files/1.4919398.pdf$$yOpenAccess
000220265 8564_ $$uhttps://bib-pubdb1.desy.de/record/220265/files/1.4919398.gif?subformat=icon$$xicon$$yOpenAccess
000220265 8564_ $$uhttps://bib-pubdb1.desy.de/record/220265/files/1.4919398.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000220265 8564_ $$uhttps://bib-pubdb1.desy.de/record/220265/files/1.4919398.jpg?subformat=icon-700$$xicon-700$$yOpenAccess
000220265 8564_ $$uhttps://bib-pubdb1.desy.de/record/220265/files/1.4919398.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000220265 909CO $$ooai:bib-pubdb1.desy.de:220265$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000220265 9101_ $$0I:(DE-588b)16047298-2$$6P:(DE-H253)PIP1013871$$aThe European X-Ray Laser Project XFEL$$b0$$kXFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1012452$$aDeutsches Elektronen-Synchrotron$$b1$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1012452$$aCentre for Free-Electron Laser Science$$b1$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1006443$$aDeutsches Elektronen-Synchrotron$$b3$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1006443$$aCentre for Free-Electron Laser Science$$b3$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1008245$$aDeutsches Elektronen-Synchrotron$$b4$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1008245$$aCentre for Free-Electron Laser Science$$b4$$kCFEL
000220265 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1016993$$aEuropean XFEL$$b5$$kXFEL.EU
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1016189$$aDeutsches Elektronen-Synchrotron$$b7$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1016189$$aCentre for Free-Electron Laser Science$$b7$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1011740$$aDeutsches Elektronen-Synchrotron$$b8$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1011740$$aCentre for Free-Electron Laser Science$$b8$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1011659$$aDeutsches Elektronen-Synchrotron$$b11$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1011659$$aCentre for Free-Electron Laser Science$$b11$$kCFEL
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1017962$$aCentre for Free-Electron Laser Science$$b12$$kCFEL
000220265 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1017962$$aEuropäisches Laboratorium für Molekularbiologie$$b12$$kEMBL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1012087$$aDeutsches Elektronen-Synchrotron$$b18$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1012087$$aCentre for Free-Electron Laser Science$$b18$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1011192$$aDeutsches Elektronen-Synchrotron$$b21$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1011192$$aCentre for Free-Electron Laser Science$$b21$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1016300$$aDeutsches Elektronen-Synchrotron$$b22$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1016300$$aCentre for Free-Electron Laser Science$$b22$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1010779$$aDeutsches Elektronen-Synchrotron$$b23$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1010779$$aCentre for Free-Electron Laser Science$$b23$$kCFEL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1006155$$aDeutsches Elektronen-Synchrotron$$b24$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1006155$$aCentre for Free-Electron Laser Science$$b24$$kCFEL
000220265 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1006155$$aEuropäisches Laboratorium für Molekularbiologie$$b24$$kEMBL
000220265 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1006324$$aDeutsches Elektronen-Synchrotron$$b26$$kDESY
000220265 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1006324$$aCentre for Free-Electron Laser Science$$b26$$kCFEL
000220265 9131_ $$0G:(DE-HGF)POF3-621$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6211$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vIn-house research on the structure, dynamics and function of matter$$x0
000220265 9141_ $$y2015
000220265 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000220265 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000220265 9201_ $$0I:(DE-H253)FS-CFEL-3-20120731$$kFS-CFEL-3$$lCFEL-Theory$$x0
000220265 980__ $$ajournal
000220265 980__ $$aVDB
000220265 980__ $$aI:(DE-H253)FS-CFEL-3-20120731
000220265 980__ $$aUNRESTRICTED
000220265 9801_ $$aFullTexts