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@ARTICLE{Pogenberg:207457,
author = {Pogenberg, Vivian and Consani Textor, Larissa and
Vanhille, Laurent and Holton, Simon J. and Sieweke,
Michael H. and Wilmanns, Matthias},
title = {{D}esign of a b{Z}ip {T}ranscription {F}actor with
{H}omo/{H}eterodimer-{I}nduced {DNA}-{B}inding {P}reference},
journal = {Structure},
volume = {22},
number = {3},
issn = {0969-2126},
address = {London [u.a.]},
publisher = {Elsevier Science},
reportid = {PUBDB-2015-01355},
pages = {466 - 477},
year = {2014},
note = {© Elsevier Ltd. Open Archive},
abstract = {The ability of basic leucine zipper transcription factors
for homo- or heterodimerization provides a paradigm for
combinatorial control of eukaryotic gene expression. It has
been unclear, however, how facultative dimerization results
in alternative DNA-binding repertoires on distinct
regulatory elements. To unravel the molecular basis of such
coupled preferences, we determined two high-resolution
structures of the transcription factor MafB as a homodimer
and as a heterodimer with c-Fos bound to variants of the
Maf-recognition element. The structures revealed several
unexpected and dimer-specific coiled-coil-heptad
interactions. Based on these findings, we have engineered
two MafB mutants with opposite dimerization preferences. One
of them showed a strong preference for MafB/c-Fos
heterodimerization and enabled selection of
heterodimer-favoring over homodimer-specific Maf-recognition
element variants. Our data provide a concept for
transcription factor design to selectively activate
dimer-specific pathways and binding repertoires.},
cin = {EMBL},
ddc = {570},
cid = {I:(DE-H253)EMBL-20120731},
pnm = {DORIS Beamline BW7 (POF2-54G13) / DORIS Beamline K1.3
(POF2-54G13)},
pid = {G:(DE-H253)POF2-BW7-20130405 /
G:(DE-H253)POF2-K1.3-20130405},
experiment = {EXP:(DE-H253)D-BW7-20150101 / EXP:(DE-H253)D-K1.3-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000333025700012},
pubmed = {pmid:24530283},
doi = {10.1016/j.str.2013.12.017},
url = {https://bib-pubdb1.desy.de/record/207457},
}