TY  - JOUR
AU  - Pogenberg, Vivian
AU  - Consani Textor, Larissa
AU  - Vanhille, Laurent
AU  - Holton, Simon J.
AU  - Sieweke, Michael H.
AU  - Wilmanns, Matthias
TI  - Design of a bZip Transcription Factor with Homo/Heterodimer-Induced DNA-Binding Preference
JO  - Structure
VL  - 22
IS  - 3
SN  - 0969-2126
CY  - London [u.a.]
PB  - Elsevier Science
M1  - PUBDB-2015-01355
SP  - 466 - 477
PY  - 2014
N1  - © Elsevier Ltd. Open Archive 
AB  - The ability of basic leucine zipper transcription factors for homo- or heterodimerization provides a paradigm for combinatorial control of eukaryotic gene expression. It has been unclear, however, how facultative dimerization results in alternative DNA-binding repertoires on distinct regulatory elements. To unravel the molecular basis of such coupled preferences, we determined two high-resolution structures of the transcription factor MafB as a homodimer and as a heterodimer with c-Fos bound to variants of the Maf-recognition element. The structures revealed several unexpected and dimer-specific coiled-coil-heptad interactions. Based on these findings, we have engineered two MafB mutants with opposite dimerization preferences. One of them showed a strong preference for MafB/c-Fos heterodimerization and enabled selection of heterodimer-favoring over homodimer-specific Maf-recognition element variants. Our data provide a concept for transcription factor design to selectively activate dimer-specific pathways and binding repertoires.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000333025700012
C6  - pmid:24530283
DO  - DOI:10.1016/j.str.2013.12.017
UR  - https://bib-pubdb1.desy.de/record/207457
ER  -