%0 Journal Article
%A Pogenberg, Vivian
%A Consani Textor, Larissa
%A Vanhille, Laurent
%A Holton, Simon J.
%A Sieweke, Michael H.
%A Wilmanns, Matthias
%T Design of a bZip Transcription Factor with Homo/Heterodimer-Induced DNA-Binding Preference
%J Structure
%V 22
%N 3
%@ 0969-2126
%C London [u.a.]
%I Elsevier Science
%M PUBDB-2015-01355
%P 466 - 477
%D 2014
%Z © Elsevier Ltd. Open Archive 
%X The ability of basic leucine zipper transcription factors for homo- or heterodimerization provides a paradigm for combinatorial control of eukaryotic gene expression. It has been unclear, however, how facultative dimerization results in alternative DNA-binding repertoires on distinct regulatory elements. To unravel the molecular basis of such coupled preferences, we determined two high-resolution structures of the transcription factor MafB as a homodimer and as a heterodimer with c-Fos bound to variants of the Maf-recognition element. The structures revealed several unexpected and dimer-specific coiled-coil-heptad interactions. Based on these findings, we have engineered two MafB mutants with opposite dimerization preferences. One of them showed a strong preference for MafB/c-Fos heterodimerization and enabled selection of heterodimer-favoring over homodimer-specific Maf-recognition element variants. Our data provide a concept for transcription factor design to selectively activate dimer-specific pathways and binding repertoires.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000333025700012
%$ pmid:24530283
%R 10.1016/j.str.2013.12.017
%U https://bib-pubdb1.desy.de/record/207457