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@ARTICLE{Shimanovskaya:207425,
author = {Shimanovskaya, Ekaterina and Viscardi, Valeria and
Lesigang, Johannes and Lettman, Molly M. and Qiao, Renping
and Svergun, Dmitri and Round, Adam and Oegema, Karen and
Dong, Gang},
title = {{S}tructure of the {C}. {E}legans {ZYG}-1 {C}ryptic {P}olo
{B}ox {S}uggests a {C}onserved {M}echanism for {C}entriolar
{D}ocking of {P}lk4 {K}inases},
journal = {Structure},
volume = {22},
number = {8},
issn = {0969-2126},
address = {London [u.a.]},
publisher = {Elsevier Science},
reportid = {PUBDB-2015-01335},
pages = {1090 - 1104},
year = {2014},
note = {(c) Elsevier Ltd. Post referee full text in progress.},
abstract = {Plk4 family kinases control centriole assembly. Plk4s
target mother centrioles through an interaction between
their cryptic polo box (CPB) and acidic regions in the
centriolar receptors SPD-2/Cep192 and/or Asterless/Cep152.
Here, we report a crystal structure for the CPB of C.
elegans ZYG-1, which forms a Z-shaped dimer containing an
intermolecular β sheet with an extended basic surface
patch. Biochemical and in vivo analysis revealed that
electrostatic interactions dock the ZYG-1 CPB basic patch
onto the SPD-2-derived acidic region to promote ZYG-1
targeting and new centriole assembly. Analysis of a
different crystal form of the Drosophila Plk4 (DmPlk4) CPB
suggests that it also forms a Z-shaped dimer. Comparison of
the ZYG-1 and DmPlk4 CPBs revealed structural changes in the
ZYG-1 CPB that confer selectivity for binding SPD-2 over
Asterless-derived acidic regions. Overall, our findings
suggest a conserved mechanism for centriolar docking of Plk4
homologs that initiate daughter centriole assembly.},
cin = {EMBL / DOOR},
ddc = {570},
cid = {I:(DE-H253)EMBL-20120731 / I:(DE-H253)HAS-User-20120731},
pnm = {DORIS Beamline D1.2 (POF2-54G13)},
pid = {G:(DE-H253)POF2-D1.2-20130405},
experiment = {EXP:(DE-H253)D-D1.2-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000340488100004},
pubmed = {pmid:24980795},
doi = {10.1016/j.str.2014.05.009},
url = {https://bib-pubdb1.desy.de/record/207425},
}