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@ARTICLE{Soykan:207421,
author = {Soykan, T. and Schneeberger, D. and Tria, G. and Buechner,
C. and Bader, N. and Svergun, D. and Tessmer, I. and
Poulopoulos, A. and Papadopoulos, T. and Varoqueaux, F. and
Schindelin, H. and Brose, N.},
title = {{A} {C}onformational {S}witch in {C}ollybistin {D}etermines
the {D}ifferentiation of {I}nhibitory {P}ostsynapses},
journal = {The EMBO journal},
volume = {33},
number = {18},
issn = {1460-2075},
address = {Heidelberg},
publisher = {EMBO Press},
reportid = {PUBDB-2015-01331},
pages = {2113 - 2133},
year = {2014},
note = {(c) The Authors},
abstract = {The formation of neuronal synapses and the dynamic
regulation of their efficacy depend on the assembly of the
postsynaptic neurotransmitter receptor apparatus. Receptor
recruitment to inhibitory GABAergic and glycinergic synapses
is controlled by the scaffold protein gephyrin and the
adaptor protein collybistin. We derived new insights into
the structure of collybistin and used these to design
biochemical, cell biological, and genetic analyses of
collybistin function. Our data define a collybistin‐based
protein interaction network that controls the gephyrin
content of inhibitory postsynapses. Within this network,
collybistin can adopt open/active and closed/inactive
conformations to act as a switchable adaptor that links
gephyrin to plasma membrane phosphoinositides. This function
of collybistin is regulated by binding of the adhesion
protein neuroligin‐2, which stabilizes the open/active
conformation of collybistin at the postsynaptic plasma
membrane by competing with an intramolecular interaction in
collybistin that favors the closed/inactive conformation. By
linking trans‐synaptic neuroligin‐dependent adhesion and
phosphoinositide signaling with gephyrin recruitment, the
collybistin‐based regulatory switch mechanism represents
an integrating regulatory node in the formation and function
of inhibitory postsynapses.},
cin = {EMBL / EMBL-User},
ddc = {570},
cid = {I:(DE-H253)EMBL-20120731 / I:(DE-H253)EMBL-User-20120814},
pnm = {DORIS Beamline D1.2 (POF2-54G13) / EUROSPIN - European
Consortium on Synaptic Protein Networks in Neurological and
Psychiatric Diseases (241498) / SYNSYS - Synaptic Systems:
dissecting brain function in health and disease (242167) /
BIOSTRUCT-X - Transnational access and enhancement of
integrated Biological Structure determination at synchrotron
X-ray radiation facilities (283570)},
pid = {G:(DE-H253)POF2-D1.2-20130405 / G:(EU-Grant)241498 /
G:(EU-Grant)242167 / G:(EU-Grant)283570},
experiment = {EXP:(DE-H253)D-D1.2-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000342503000012},
pubmed = {pmid:25082542},
doi = {10.15252/embj.201488143},
url = {https://bib-pubdb1.desy.de/record/207421},
}