TY  - JOUR
AU  - Kaljunen, Heidi
AU  - Chow, Jennifer
AU  - Streit, Wolfgang R.
AU  - Mueller-Dieckmann, Jochen
TI  - Cloning, Expression, Purification and Preliminary X-ray Analysis of EstN2, a Novel Archaeal α/β-Hydrolase from Candidatus Nitrososphaera Gargensis
JO  - Acta crystallographica / F
VL  - 70
SN  - 1744-3091
CY  - Oxford [u.a.]
PB  - Blackwell
M1  - PUBDB-2015-01329
SP  - 1394-1397
PY  - 2014
N1  - (c) International Union of Crystallography. Post referee full text in progress.
AB  - EstN2 is a novel α/β-hydrolase originating from the ammonia-oxidizing thaumarchaeon <i>Candidatus</i> Nitrososphaera gargensis. The genome of the organism was sequenced and genes conferring putative lipolytic activity were amplified and cloned into <i>Escherichia</i> <i>coli</i> as a heterologous host. Through function-based screening, esterase and lipase activity was detected. A recombinant enzyme designated EstN2 was successfully expressed, purified and crystallized. The crystals belonged to space group <i>I</i>2, with one molecule per asymmetric unit, and diffracted X-rays to 1.5 Å resolution.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000343060200018
C6  - pmid:25286947
DO  - DOI:10.1107/S2053230X14018482
UR  - https://bib-pubdb1.desy.de/record/207419
ER  -