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000207419 1001_ $$0P:(DE-H253)PIP1017942$$aKaljunen, Heidi$$b0
000207419 245__ $$aCloning, Expression, Purification and Preliminary X-ray Analysis of EstN2, a Novel Archaeal $\alpha/\beta$-Hydrolase from Candidatus Nitrososphaera Gargensis
000207419 260__ $$aOxford [u.a.]$$bBlackwell$$c2014
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000207419 520__ $$aEstN2 is a novel $\alpha/\beta$-hydrolase originating from the ammonia-oxidizing thaumarchaeon $\mathit{Candidatus}$ Nitrososphaera gargensis. The genome of the organism was sequenced and genes conferring putative lipolytic activity were amplified and cloned into $\mathit{Escherichia coli}$ as a heterologous host. Through function-based screening, esterase and lipase activity was detected. A recombinant enzyme designated EstN2 was successfully expressed, purified and crystallized. The crystals belonged to space group $\mathit{I2}$, with one molecule per asymmetric unit, and diffracted X-rays to 1.5 Å resolution.
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000207419 7001_ $$0P:(DE-HGF)0$$aChow, Jennifer$$b1
000207419 7001_ $$0P:(DE-HGF)0$$aStreit, Wolfgang R.$$b2
000207419 7001_ $$0P:(DE-HGF)0$$aMueller-Dieckmann, Jochen$$b3$$eCorresponding author
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