%0 Journal Article
%A Kaljunen, Heidi
%A Chow, Jennifer
%A Streit, Wolfgang R.
%A Mueller-Dieckmann, Jochen
%T Cloning, Expression, Purification and Preliminary X-ray Analysis of EstN2, a Novel Archaeal α/β-Hydrolase from Candidatus Nitrososphaera Gargensis
%J Acta crystallographica / F
%V 70
%@ 1744-3091
%C Oxford [u.a.]
%I Blackwell
%M PUBDB-2015-01329
%P 1394-1397
%D 2014
%Z (c) International Union of Crystallography. Post referee full text in progress.
%X EstN2 is a novel α/β-hydrolase originating from the ammonia-oxidizing thaumarchaeon <i>Candidatus</i> Nitrososphaera gargensis. The genome of the organism was sequenced and genes conferring putative lipolytic activity were amplified and cloned into <i>Escherichia</i> <i>coli</i> as a heterologous host. Through function-based screening, esterase and lipase activity was detected. A recombinant enzyme designated EstN2 was successfully expressed, purified and crystallized. The crystals belonged to space group <i>I</i>2, with one molecule per asymmetric unit, and diffracted X-rays to 1.5 Å resolution.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000343060200018
%$ pmid:25286947
%R 10.1107/S2053230X14018482
%U https://bib-pubdb1.desy.de/record/207419