TY - JOUR AU - Ribeiro, Euripedes de Almeida AU - Pinotsis, Nikos AU - Ghisleni, Andrea AU - Salmazo, Anita AU - Konarev, Petr AU - Kostan, Julius AU - Sjöblom, Björn AU - Schreiner, Claudia AU - Polyansky, Anton A. AU - Gkougkoulia, Eirini A. AU - Holt, Mark R. AU - Aachmann, Finn L. AU - Žagrović, Bojan AU - Bordignon, Enrica AU - Pirker, Katharina F. AU - Svergun, Dmitri AU - Gautel, Mathias AU - Djinović-Carugo, Kristina TI - The Structure and Regulation of Human Muscle α-Actinin JO - Cell VL - 159 IS - 6 SN - 0092-8674 CY - [Cambridge, Mass.] PB - Cell Press M1 - PUBDB-2015-01323 SP - 1447 - 1460 PY - 2014 N1 - OA AB - The spectrin superfamily of proteins plays key roles in assembling the actin cytoskeleton in various cell types, crosslinks actin filaments, and acts as scaffolds for the assembly of large protein complexes involved in structural integrity and mechanosensation, as well as cell signaling. α-actinins in particular are the major actin crosslinkers in muscle Z-disks, focal adhesions, and actin stress fibers. We report a complete high-resolution structure of the 200 kDa α-actinin-2 dimer from striated muscle and explore its functional implications on the biochemical and cellular level. The structure provides insight into the phosphoinositide-based mechanism controlling its interaction with sarcomeric proteins such as titin, lays a foundation for studying the impact of pathogenic mutations at molecular resolution, and is likely to be broadly relevant for the regulation of spectrin-like proteins LB - PUB:(DE-HGF)16 UR - <Go to ISI:>//WOS:000346652900021 C6 - pmid:25433700 DO - DOI:10.1016/j.cell.2014.10.056 UR - https://bib-pubdb1.desy.de/record/207413 ER -