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000207279 1001_ $$0P:(DE-HGF)0$$aHunter, Mark S.$$b0$$eCorresponding Author
000207279 245__ $$aFixed-Target Protein Serial Microcrystallography with an X-ray Free Electron Laser
000207279 260__ $$aLondon$$bNature Publishing Group$$c2014
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000207279 520__ $$aWe present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution (~2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of ~38%, indicating that a complete data set could be acquired in about one 12-hour LCLS shift using the setup described here, or in even less time using hardware optimized for fixed target SFX. This demonstration opens the door to ultra low sample consumption SFX using the technique of diffraction-before-destruction on proteins that exist in only small quantities and/or do not produce the copious quantities of microcrystals required for flowing jet methods.
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000207279 7001_ $$0P:(DE-HGF)0$$aSegelke, Brent$$b1
000207279 7001_ $$0P:(DE-H253)PIP1005807$$aMesserschmidt, Marc$$b2
000207279 7001_ $$0P:(DE-HGF)0$$aWilliams, Garth J.$$b3
000207279 7001_ $$0P:(DE-HGF)0$$aZatsepin, Nadia A.$$b4
000207279 7001_ $$0P:(DE-H253)PIP1008245$$aBarty, Anton$$b5
000207279 7001_ $$0P:(DE-HGF)0$$aBenner, W. Henry$$b6
000207279 7001_ $$0P:(DE-HGF)0$$aCarlson, David B.$$b7
000207279 7001_ $$0P:(DE-HGF)0$$aColeman, Matthew$$b8
000207279 7001_ $$0P:(DE-H253)PIP1015138$$aGraf, Alexander$$b9
000207279 7001_ $$0P:(DE-HGF)0$$aHau-Riege, Stefan P.$$b10
000207279 7001_ $$0P:(DE-HGF)0$$aPardini, Tommaso$$b11
000207279 7001_ $$0P:(DE-HGF)0$$aSeibert, M. Marvin$$b12
000207279 7001_ $$0P:(DE-HGF)0$$aEvans, James$$b13
000207279 7001_ $$0P:(DE-H253)PIP1009086$$aBoutet, Sébastien$$b14
000207279 7001_ $$0P:(DE-H253)PIP1009688$$aFrank, Matthias$$b15
000207279 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/srep06026$$gVol. 4$$n6026$$p1$$tScientific reports$$v4$$x2045-2322$$y20142014
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