%0 Journal Article
%A Hunter, Mark S.
%A Segelke, Brent
%A Messerschmidt, Marc
%A Williams, Garth J.
%A Zatsepin, Nadia A.
%A Barty, Anton
%A Benner, W. Henry
%A Carlson, David B.
%A Coleman, Matthew
%A Graf, Alexander
%A Hau-Riege, Stefan P.
%A Pardini, Tommaso
%A Seibert, M. Marvin
%A Evans, James
%A Boutet, Sébastien
%A Frank, Matthias
%T Fixed-Target Protein Serial Microcrystallography with an X-ray Free Electron Laser
%J Scientific reports
%V 4
%N 6026
%@ 2045-2322
%C London
%I Nature Publishing Group
%M PUBDB-2015-01262
%P 1
%D 2014
%Z OA
%X We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution ( 2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of  38
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000340674000004
%$ pmid:25113598
%R 10.1038/srep06026
%U https://bib-pubdb1.desy.de/record/207279