%0 Journal Article
%A Hellert, Jan
%A Krausze, Joern
%A Schulz, Thomas F.
%A Lührs, Thorsten
%T Crystallization, room-temperature X-ray diffraction and preliminary analysis of Kaposi's sarcoma herpesvirus LANA bound to DNA
%J Acta crystallographica / F
%V 70
%N Pt11
%@ 1744-3091
%C Oxford [u.a.]
%I Blackwell
%M PUBDB-2015-00294
%P 1570-1574
%D 2014
%X The latency-associated nuclear antigen (LANA) is the latent origin-binding protein and chromatin anchor of the Kaposi's sarcoma herpesvirus (KSHV/HHV-8) genome. Its C-terminal domain (CTD) binds sequence-specifically to the viral origin of replication, whereas the N-terminal domain links it to nucleosomes of cellular chromatin for long-term persistence in dividing host cells. Here, the crystallization and X-ray data acquisition of a mutant LANA CTD in complex with its wild-type target DNA LBS1 is described. This report describes the rational protein engineering for successful co-crystallization with DNA and X-ray diffraction data collection at room temperature on the high-brilliance third-generation synchrotron PETRA III at DESY, Germany.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000344790900025
%R 10.1107/S2053230X14019906
%U https://bib-pubdb1.desy.de/record/205734