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@ARTICLE{Lis:205640,
      author       = {Lis, Grzegorz J. and Czapla-Masztafiak, Joanna and Kwiatek,
                      Wojciech M. and Gajda, Mariusz and Jasek, Ewa and Jasinska,
                      Malgorzata and Czubek, Urszula and Borchert, Manuela and
                      Appel, Karen and Nessler, Jadwiga and Sadowski, Jerzy and
                      Litwin, Jan A.},
      title        = {{D}istribution of {S}elected {E}lements in {C}alcific
                      {H}uman {A}ortic {V}alves {S}tudied by {M}icroscopy
                      {C}ombined with {SR}-µ{XRF}: {I}nfluence of {L}ipids on
                      {P}rogression of {C}alcification},
      journal      = {Micron},
      volume       = {67},
      issn         = {0968-4328},
      address      = {New York, NY [u.a.]},
      publisher    = {Elsevier},
      reportid     = {PUBDB-2015-00200},
      pages        = {141 - 148},
      year         = {2014},
      note         = {(c) Elsevier Ltd. Post referee fulltext in progress.},
      abstract     = {Calcified heart valves display a significant imbalance in
                      tissue content of trace and essential elements. The valvular
                      calcification is an age-related process and there are data
                      suggesting involvement of lipids. We studied elemental
                      composition and lipid distribution in three distinct regions
                      of calcified human aortic valves, representing successive
                      stages of the calcific degeneration: normal, thickened
                      (early lesion) and calcified (late lesion), using $SR- \mu
                      XRF$ (Synchrotron Radiation Micro X-Ray Fluorescence) for
                      elemental composition and Oil Red O (ORO) staining for
                      demonstration of lipids. Two-dimensional $SR- \mu XRF$ maps
                      and precise point spectra were compared with histological
                      stainings on consecutive valve sections to prove
                      topographical localization and colocalization of the
                      examined elements and lipids. In calcified valve areas,
                      accumulation of calcium and phosphorus was accompanied by
                      enhanced concentrations of strontium and zinc.
                      Calcifications preferentially developed in lipid-rich areas
                      of the valves. Calcium concentration ratio between
                      lipid-rich and lipid-free areas was not age-dependent in
                      early lesions, but showed a significant increase with age in
                      late lesions, indicating age-dependent intensification of
                      lipid involvement in calcification process. The results
                      suggest that mechanisms of calcification change with
                      progression of valve degeneration and with age.},
      cin          = {DOOR / FS-DO},
      ddc          = {570},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)FS-DO-20120731},
      pnm          = {DORIS Beamline L (POF2-54G13) / FS-Proposal: II-20100089 EC
                      (II-20100089-EC)},
      pid          = {G:(DE-H253)POF2-L-20130405 / G:(DE-H253)II-20100089-EC},
      experiment   = {EXP:(DE-H253)D-L-20150101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000343641300018},
      pubmed       = {pmid:25169034},
      doi          = {10.1016/j.micron.2014.08.002},
      url          = {https://bib-pubdb1.desy.de/record/205640},
}