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@ARTICLE{Brennich:192723,
author = {Brennich, Martha and Bauch, Susanne and Vainio, Ulla and
Wedig, Tatjana and Herrmann, Harald and Koester, Sarah},
title = {{I}mpact of ion valency on the assembly of vimentin studied
by quantitative small angle {X}-ray scattering},
journal = {Soft matter},
volume = {10},
issn = {1744-683X},
address = {London},
publisher = {Royal Soc. of Chemistry},
reportid = {PUBDB-2014-04262},
pages = {2059-2068},
year = {2014},
note = {(c) The Royal Society of Chemistry. Code P.},
abstract = {The assembly kinetics of intermediate filament (IF)
proteins from tetrameric complexes to single filaments and
networks depends on the protein concentration, temperature
and the ionic composition of their environment. We
systematically investigate how changes in the concentration
of monovalent potassium and divalent magnesium ions affect
the internal organization of the resulting filaments. Small
angle X-ray scattering (SAXS) is very sensitive to changes
in the filament cross-section such as diameter or
compactness. Our measurements reveal that filaments formed
in the presence of magnesium chloride differ distinctly from
filaments formed in the presence of potassium chloride. The
principle multi-step assembly mechanism from tetramers via
unit-length filaments (ULF) to elongated filaments is not
changed by the valency of ions. However, the observed
differences indicate that the magnesium ions free the head
domains of tetramers from unproductive interactions to allow
assembly but at the same time mediate strong
inter-tetrameric interactions that impede longitudinal
annealing of unit-length filaments considerably, thus
slowing down filament growth.},
cin = {DOOR},
ddc = {530},
cid = {I:(DE-H253)HAS-User-20120731},
pnm = {DORIS Beamline B1 (POF2-54G13) / FS-Proposal: I-20100163
(I-20100163)},
pid = {G:(DE-H253)POF2-B1-20130405 / G:(DE-H253)I-20100163},
experiment = {EXP:(DE-H253)D-B1-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000332463300021},
pubmed = {pmid:24800271},
doi = {10.1039/C3SM52532E},
url = {https://bib-pubdb1.desy.de/record/192723},
}