TY  - JOUR
AU  - Piotto, Stefano
AU  - Concilio, Simona
AU  - Bianchino, Erminia
AU  - Iannelli, Pio
AU  - López, David J.
AU  - Terés, Silvia
AU  - Ibarguren, Maitane
AU  - Barceló-Coblijn, Gwendolyn
AU  - Martin, Maria Laura
AU  - Guardiola-Serrano, Francisca
AU  - Alonso-Sande, María
AU  - Funari, Sérgio S.
AU  - Busquets, Xavier
AU  - Escribá, Pablo V.
TI  - Differential effect of 2-hydroxyoleic acid enantiomers on protein (sphingomyelin synthase) and lipid (membrane) targets
JO  - Biochimica et biophysica acta / Biomembranes
VL  - 1838
IS  - 6
SN  - 0005-2736
CY  - Amsterdam
PB  - Elsevier
M1  - PUBDB-2014-04165
SP  - 1628 - 1637
PY  - 2014
AB  - The complex dual mechanism of action of 2-hydroxyoleic acid (2OHOA), a potent anti-tumor compound used in membrane lipid therapy (MLT), has yet to be fully elucidated. It has been demonstrated that 2OHOA increases the sphingomyelin (SM) cell content via SM synthase (SGMS) activation. Its presence in membranes provokes changes in the membrane lipid structure that induce the translocation of PKC to the membrane and the subsequent overexpression of CDK inhibitor proteins (e.g., p21(Cip1)). In addition, 2OHOA also induces the translocation of Ras to the cytoplasm, provoking the silencing of MAPK and its related pathways. These two differential modes of action are triggered by the interactions of 2OHOA with either lipids or proteins. To investigate the molecular basis of the different interactions of 2OHOA with membrane lipids and proteins, we synthesized the R and S enantiomers of this compound. A molecular dynamics study indicated that both enantiomers interact similarly with lipid bilayers, which was further confirmed by X-ray diffraction studies. By contrast, only the S enantiomer was able to activate SMS in human glioma U118 cells. Moreover, the anti-tumor efficacy of the S enantiomer was greater than that of the R enantiomer, as the former can act through both MLT mechanisms. The present study provides additional information on this novel therapeutic approach and on the magnitude of the therapeutic effects of type-1 and type-2 MLT approaches. This article is part of a Special Issue entitled: Membrane Structure and Function: Relevance in the Cell's Physiology, Pathology and Therapy.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000335619200017
C6  - pmid:24412218
DO  - DOI:10.1016/j.bbamem.2013.12.023
UR  - https://bib-pubdb1.desy.de/record/192583
ER  -