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@ARTICLE{Kamiski:191812,
author = {Kamiński, Daniel M. and Czernel, Grzegorz and Murphy,
Bridget and Runge, Benjamin and Magnussen, Olaf M. and
Gagoś, Mariusz},
title = {{E}ffect of cholesterol and ergosterol on the antibiotic
amphotericin {B} interactions with
dipalmitoylphosphatidylcholine monolayers: {X}-ray
reflectivity study},
journal = {Biochimica et biophysica acta / Biomembranes},
volume = {1838},
number = {11},
issn = {0005-2736},
address = {Amsterdam},
publisher = {Elsevier},
reportid = {PUBDB-2014-04000},
pages = {2947 - 2953},
year = {2014},
abstract = {Amphotericin B is a Streptomyces nodosus metabolite and one
of the oldest polyene antibiotics used in the treatment of
invasive systemic fungal infections. Despite its over
50-year existence in clinical practice and the recognition
of amphotericin B as the gold standard in the treatment of
serious systemic mycosis, it still remains one of the most
toxic pharmaceuticals. Understanding of the processes at the
molecular levels and the interactions between amphotericin B
with lipid membranes containing sterols should elucidate the
mechanisms of the action and toxicity of this widely used
antibiotic. In this work, we use X-ray reflectivity to study
the structural changes on a molecular scale after
amphotericin B incorporation. These changes are accompanied
by an increase in monolayer surface pressure which is more
pronounced for ergosterol — rather than cholesterol-rich
membranes. The data indicate that this difference is not due
to the higher affinity of amphotericin B towards
ergosterol-containing membranes but is rather due to a ~ 3
Angstrom corrugation of the monolayer. Furthermore, the
total quantity of amphotericin B incorporated into lipid
monolayers containing cholesterol and ergosterol is the
same.},
cin = {DOOR},
ddc = {570},
cid = {I:(DE-H253)HAS-User-20120731},
pnm = {PETRA Beamline P08 (POF2-54G14)},
pid = {G:(DE-H253)POF2-P08-20130405},
experiment = {EXP:(DE-H253)P-P08-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000342477400020},
pubmed = {pmid:25128151},
doi = {10.1016/j.bbamem.2014.08.004},
url = {https://bib-pubdb1.desy.de/record/191812},
}