TY  - JOUR
AU  - Hellmich, Julia
AU  - Bommer, Martin
AU  - Burkhardt, Anja
AU  - Ibrahim, Mohamed
AU  - Kern, Jan
AU  - Meents, Alke
AU  - Müh, Frank
AU  - Dobbek, Holger
AU  - Zouni, Athina
TI  - Native-like Photosystem II Superstructure at 2.44 Å Resolution through Detergent Extraction from the Protein Crystal
JO  - Structure
VL  - 22
IS  - 11
SN  - 0969-2126
CY  - London [u.a.]
PB  - Elsevier Science
M1  - PUBDB-2014-03951
SP  - 1607 - 1615
PY  - 2014
N1  - OA
AB  - Photosystem II (PSII) catalyzes a key step in photosynthesis, the oxidation of water to oxygen. Excellent structural models exist for the dimeric PSII core complex of cyanobacteria, but higher order physiological assemblies readily dissociate when solubilized from the native thylakoid membrane with detergent. Here, we describe the crystallization of PSII from Thermosynechococcus elongatus with a postcrystallization treatment involving extraction of the detergent C12E8. This resulted in a transition from Type II to Type I-like membrane protein crystals and improved diffraction to 2.44 Å resolution. The obtained PSII packing in precise rows, interconnected by specific pairs of galactolipids and a loop in the PsbO subunit specific to cyanobacteria, is superimposable with previous electron microscopy images of the thylakoid membrane. The study provides a detailed model of such a superstructure and its organization of light-harvesting pigments with possible implications for the understanding of their efficient use of solar energy.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000344934300008
C6  - pmid:25438669
DO  - DOI:10.1016/j.str.2014.09.007
UR  - https://bib-pubdb1.desy.de/record/191761
ER  -