000168905 001__ 168905
000168905 005__ 20250730143956.0
000168905 0247_ $$2doi$$a10.1107/S1399004713032446
000168905 0247_ $$2ISSN$$a0907-4449
000168905 0247_ $$2ISSN$$a1399-0047
000168905 0247_ $$2WOS$$aWOS:000332406600010
000168905 0247_ $$2pmid$$apmid:24598740
000168905 0247_ $$2altmetric$$aaltmetric:2123608
000168905 0247_ $$2openalex$$aopenalex:W2130084401
000168905 037__ $$aDESY-2014-02720
000168905 082__ $$a570
000168905 1001_ $$0P:(DE-HGF)0$$aPonomarenko, Natalia$$b0$$eCorresponding Author
000168905 245__ $$aRole of κ→λ light-chain constant-domain switch in the structure and functionality of A17 reactibody
000168905 260__ $$aCopenhagen$$bMunksgaard$$c2014
000168905 3367_ $$2DRIVER$$aarticle
000168905 3367_ $$2DataCite$$aOutput Types/Journal article
000168905 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1605000473_18976
000168905 3367_ $$2BibTeX$$aARTICLE
000168905 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000168905 3367_ $$00$$2EndNote$$aJournal Article
000168905 500__ $$3POF3_Assignment on 2016-02-10
000168905 520__ $$aThe engineering of catalytic function in antibodies requires precise information on their structure. Here, results are presented that show how the antibody domain structure affects its functionality. The previously designed organophosphate-metabolizing reactibody A17 has been re-engineered by replacing its constant κ light chain by the λ chain (A17λ), and the X-ray structure of A17λ has been determined at 1.95 Å resolution. It was found that compared with A17κ the active centre of A17λ is displaced, stabilized and made more rigid owing to interdomain interactions involving the CDR loops from the VL and VH domains. These VL/VH domains also have lower mobility, as deduced from the atomic displacement parameters of the crystal structure. The antibody elbow angle is decreased to 126° compared with 138° in A17κ. These structural differences account for the subtle changes in catalytic efficiency and thermodynamic parameters determined with two organophosphate ligands, as well as in the affinity for peptide substrates selected from a combinatorial cyclic peptide library, between the A17κ and A17λ variants. The data presented will be of interest and relevance to researchers dealing with the design of antibodies with tailor-made functions.
000168905 536__ $$0G:(DE-H253)POF2-P14-20130405$$aPETRA Beamline P14 (POF2-54G14)$$cPOF2-54G14$$fPOF II$$x0
000168905 588__ $$aDataset connected to CrossRef, bib-pubdb1.desy.de
000168905 693__ $$0EXP:(DE-H253)P-P14-20150101$$1EXP:(DE-H253)PETRAIII-20150101$$6EXP:(DE-H253)P-P14-20150101$$aPETRA III$$fPETRA Beamline P14$$x0
000168905 7001_ $$0P:(DE-H253)PIP1094717$$aChatziefthymiou, Spyridon$$b1$$udesy
000168905 7001_ $$0P:(DE-H253)PIP1016968$$aKurkova, Inna$$b2
000168905 7001_ $$0P:(DE-HGF)0$$aMokrushina, Yuliana$$b3
000168905 7001_ $$0P:(DE-HGF)0$$aStepanova, Anastasiya$$b4
000168905 7001_ $$0P:(DE-HGF)0$$aSmirnov, Ivan$$b5
000168905 7001_ $$0P:(DE-HGF)0$$aAvakyan, Marat$$b6
000168905 7001_ $$0P:(DE-HGF)0$$aBobik, Tatyana$$b7
000168905 7001_ $$0P:(DE-HGF)0$$aMamedov, Azad$$b8
000168905 7001_ $$0P:(DE-HGF)0$$aMitkevich, Vladimir$$b9
000168905 7001_ $$0P:(DE-HGF)0$$aBelogurov, Alexey$$b10
000168905 7001_ $$0P:(DE-HGF)0$$aFedorova, Olga S.$$b11
000168905 7001_ $$0P:(DE-HGF)0$$aDubina, Michael$$b12
000168905 7001_ $$0P:(DE-HGF)0$$aGolovin, Andrey$$b13
000168905 7001_ $$0P:(DE-H253)PIP1002108$$aLamzin, Victor$$b14
000168905 7001_ $$0P:(DE-HGF)0$$aFriboulet, Alain$$b15
000168905 7001_ $$0P:(DE-HGF)0$$aMakarov, Alexander A.$$b16
000168905 7001_ $$0P:(DE-H253)PIP1001283$$aWilmanns, Matthias$$b17
000168905 7001_ $$0P:(DE-HGF)0$$aGabibov, Alexander$$b18
000168905 773__ $$0PERI:(DE-600)2020492-9$$a10.1107/S1399004713032446$$gVol. 70, no. 3, p. 708 - 719$$n3$$p708 - 719$$tActa crystallographica / D$$v70$$x1399-0047$$y2014
000168905 8564_ $$uhttps://bib-pubdb1.desy.de/record/168905/files/DESY-2014-02720.pdf$$yOpenAccess
000168905 8564_ $$uhttps://bib-pubdb1.desy.de/record/168905/files/DESY-2014-02720.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000168905 8564_ $$uhttps://bib-pubdb1.desy.de/record/168905/files/DESY-2014-02720.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000168905 8564_ $$uhttps://bib-pubdb1.desy.de/record/168905/files/DESY-2014-02720.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000168905 909CO $$ooai:bib-pubdb1.desy.de:168905$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000168905 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1094717$$aDeutsches Elektronen-Synchrotron$$b1$$kDESY
000168905 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1016968$$aExternes Institut$$b2$$kExtern
000168905 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1002108$$aEuropäisches Laboratorium für Molekularbiologie$$b14$$k>EMBL
000168905 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1002108$$aExternes Institut$$b14$$kExtern
000168905 9101_ $$0I:(DE-588b)235011-7$$6P:(DE-H253)PIP1001283$$aEuropäisches Laboratorium für Molekularbiologie$$b17$$k>EMBL
000168905 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1001283$$aExternes Institut$$b17$$kExtern
000168905 9132_ $$0G:(DE-HGF)POF3-622$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$9G:(DE-HGF)POF3-6G3$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vFacility topic: Research on Matter with Brilliant Light Sources$$x0
000168905 9131_ $$0G:(DE-HGF)POF2-54G14$$1G:(DE-HGF)POF2-540$$2G:(DE-HGF)POF2-500$$3G:(DE-HGF)POF2$$4G:(DE-HGF)POF$$9G:(DE-H253)POF2-P14-20130405$$aDE-H253$$bStruktur der Materie$$lForschung mit Photonen, Neutronen, Ionen$$vPETRA III$$x0
000168905 9141_ $$y2014
000168905 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000168905 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000168905 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000168905 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000168905 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000168905 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000168905 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000168905 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000168905 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000168905 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000168905 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000168905 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000168905 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000168905 9201_ $$0I:(DE-H253)EMBL-User-20120814$$kEMBL-User$$lEMBL-User$$x0
000168905 9201_ $$0I:(DE-H253)EMBL-20120731$$kEMBL$$lEMBL$$x1
000168905 980__ $$ajournal
000168905 980__ $$aVDB
000168905 980__ $$aI:(DE-H253)EMBL-User-20120814
000168905 980__ $$aI:(DE-H253)EMBL-20120731
000168905 980__ $$aUNRESTRICTED
000168905 9801_ $$aFullTexts