% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Thomas:166438,
      author       = {Thomas, George and Stärk, Hans-Joachim and Wellenreuther,
                      Gerd and Dickinson, Bryan C. and Kuepper, Hendrik},
      title        = {{E}ffects of nanomolar copper on water plants —
                      {C}omparison of biochemical and biophysical mechanisms of
                      deficiency and sublethal toxicity under environmentally
                      relevant conditions},
      journal      = {Aquatic toxicology},
      volume       = {140-141},
      issn         = {1879-1514},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DESY-2014-01342},
      pages        = {27–36},
      year         = {2013},
      note         = {© Elsevier B.V.; Post referee fulltext in progress;
                      Embargo 12 months from publication},
      abstract     = {Toxicity and deficiency of essential trace elements like Cu
                      are major global problems. Here, environmentally relevant
                      sub-micromolar concentrations of Cu (supplied as CuSO4) and
                      simulations of natural light- and temperature cycles were
                      applied to the aquatic macrophyte Ceratophyllum demersum.
                      Growth was optimal at 10 nM Cu, while PSII activity (Fv/Fm)
                      was maximal around 2 nM Cu. Damage to the PSII reaction
                      centre was the first target of Cu toxicity, followed by
                      disturbed regulation of heat dissipation (NPQ). Only after
                      that, electron transport through PSII (ΦPSII) was
                      inhibited, and finally chlorophylls decreased. Copper
                      accumulation in the plants was stable until 10 nM Cu in
                      solution, but strongly increased at higher concentrations.
                      The vein was the main storage site for Cu up to
                      physiological concentrations (10 nM). At toxic levels it was
                      also sequestered to the epidermis and mesophyll until export
                      from the vein became inhibited, accompanied by inhibition of
                      Zn uptake. Copper deficiency led to a complete stop of
                      growth at “0” nM Cu after 6 weeks. This was accompanied
                      by high starch accumulation although electron flow through
                      PSII (ΦPSII) decreased from 2 weeks, followed by decrease
                      in pigments and increase of non photochemical quenching
                      (NPQ). Release of Cu from the plants below 10 nM Cu supply
                      in the nutrient solution indicated lack of high-affinity Cu
                      transporters, and on the tissue level copper deficiency led
                      to a re-distribution of zinc.},
      cin          = {DOOR / FS-DO},
      ddc          = {570},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)FS-DO-20120731},
      pnm          = {DORIS Beamline L (POF2-54G13) / FS-Proposal: I-20110682
                      (I-20110682)},
      pid          = {G:(DE-H253)POF2-L-20130405 / G:(DE-H253)I-20110682},
      experiment   = {EXP:(DE-H253)D-L-20150101},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000324900800004},
      pubmed       = {pmid:23747550},
      doi          = {10.1016/j.aquatox.2013.05.008},
      url          = {https://bib-pubdb1.desy.de/record/166438},
}