TY  - JOUR
AU  - Hummer, Alfred
AU  - Rompel, Annette
TI  - The use of X-ray absorption and synchrotron based micro-X-ray fluorescence spectroscopy to investigate anti-cancer metal compounds in vivo and in vitro
JO  - Metallomics
VL  - 5
IS  - 6
SN  - 1756-591X
CY  - Cambridge
PB  - RSC Publ.
M1  - DESY-2014-01257
SP  - 597 -614
PY  - 2013
N1  - (c) The Royal Society of Chemistry; Post referee fulltext in progress; Embargo 12 months from publication
AB  - X-ray absorption spectroscopy (XAS) and micro-synchrotron based X-ray fluorescence (micro-SXRF) are element specific spectroscopic techniques and have been proven to be valuable tools for the investigation of changes in the chemical environment of metal centres. XAS allows the determination of the oxidation state, the coordination motif of the probed element, the identity and the number of adjacent atoms and the absorber–ligand distances. It is further applicable to nearly all types of samples independent of their actual physical state (solid, liquid, gaseous) down to μM concentrations. Micro-SXRF can provide information on the distribution and concentration of multiple elements within a sample simultaneously, allowing for the chemical state of several elements within subcellular compartments to be probed. Modern third generation synchrotrons offer the possibility to investigate the majority of the biologically relevant elements. The biological mode of action of metal-based compounds often involves interactions with target and/or transport molecules. The presence of reducing agents may also give rise to changes in the coordination sphere and/or the oxidation state. XAS and micro-SXRF are ideal techniques for investigating these issues. This tutorial review introduces the use of XAS and micro-SXRF techniques into the field of inorganic medicinal chemistry. The results obtained for platinum, ruthenium, gallium, gold and cobalt compounds within the last few years are presented.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000319777300002
C6  - pmid:23558305
DO  - DOI:10.1039/c3mt20261e
UR  - https://bib-pubdb1.desy.de/record/166330
ER  -