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@INPROCEEDINGS{Arcon:154275,
author = {Arcon, Iztok and Vogel-Mikus, Katarina and Kump, Peter},
title = {{M}icro-{XANES} analysis of metal accumulation in plants on
sub-cellular level},
reportid = {DESY-2013-00887},
year = {2013},
abstract = {There is a growing need to develop powerful analytical
tools for monitoring concentrations and chemical state of
trace element in the biosphere and its abiotic environment,
due to pollution and degradation of ecosystems worldwide.
Increased metal concentrations present in the environment
pose a threat to all living organisms from microorganisms
and plants to animals and humans, because they interfere
with vital biological processes. The goal is to efficiently
assess metal bioavailability and toxicity, and gain more
knowledge on the mechanisms of metal uptake, accumulation
and detoxification in living organisms [1]. In this work we
demonstrate that a combination of micro-XRF imaging and
micro-XANES and EXAFS analysis represents a powerful and
indispensable tool for characterization of metal pollutants
on subcellular level. The methodological approaches for
efficient micro-XAS experiments are presented, the
limitations and sources of potential systematic errors in
XANES and EXAFS analysis (especially at low energies) due to
self-absorption effects and strong energy dependent
penetration depth of X-ray beam in the sample are discussed.
Some typical examples of such combined micro-spectroscopy
analysis are selected from the following research fields:
Cd/Zn hyper-accumulating plants, that can be used for
phytoremediation of Cd/Zn polluted and degraded ecosystems,
including investigation of the role of externally supplied
sulphur compounds in the nutrient solution of the Cd/Zn
hyperaccumulator Thlaspi praecox, that may alter leaf Cd
distribution and Cd ligand environment [2];
biofortification, which aims to increase essential elements
(Fe) concentrations in the edible plant parts [3]; microbial
regulation of metal (Fe, Pd) uptake and formation of
Fe-oxide and Pd nanoparticles, encapsulated in
exopolysaccharide to avoid iron toxicity under anaerobic
conditions, discovered on a strain of Klebsiella oxytoca,
isolated from acid pyrite-mine drainage[4].},
month = {Sep},
date = {2013-09-02},
organization = {22nd International Congress on X-ray
Optics and Microanalysis, Hamburg
(Germany), 2 Sep 2013 - 6 Sep 2013},
subtyp = {Plenary/Keynote},
cin = {DOOR},
cid = {I:(DE-H253)HAS-User-20120731},
pnm = {DORIS Beamline C (POF2-54G13) / DORIS Beamline A1
(POF2-54G13) / FS-Proposal: I-20110511 EC (I-20110511-EC) /
CALIPSO - Coordinated Access to Lightsources to Promote
Standards and Optimization (312284)},
pid = {G:(DE-H253)POF2-C-20130405 / G:(DE-H253)POF2-A1-20130405 /
G:(DE-H253)I-20110511-EC / G:(EU-Grant)312284},
experiment = {EXP:(DE-H253)D-C-20150101 / EXP:(DE-H253)D-A1-20150101},
typ = {PUB:(DE-HGF)6},
url = {https://bib-pubdb1.desy.de/record/154275},
}