TY  - JOUR
AU  - King-scott, J.
AU  - Konarev, P. V.
AU  - Panjikar, S.
AU  - Jordanova, R.
AU  - Svergun, D. I.
AU  - Tucker, P. A.
AU  - DESY
TI  - Structural characterization of the multidomain regulatory protein Rv1364c from mycobacterium tuberculosis
JO  - Structure
VL  - 19
SN  - 0969-2126
CY  - London [u.a.]
PB  - Elsevier Science
M1  - PHPPUBDB-25737
SP  - 56
PY  - 2011
AB  - The open reading frame rv1364c of Mycobacterium tuberculosis, which regulates the stress-dependent σ factor, σ(F), has been analyzed structurally and functionally. Rv1364c contains domains with sequence similarity to the RsbP/RsbW/RsbV regulatory system of the stress-response σ factor of Bacillus subtilis. Rv1364c contains, sequentially, a PAS domain (which shows sequence similarity to the PAS domain of the B. subtilis RsbP protein), an active phosphatase domain, a kinase (anti-σ(F) like) domain and a C-terminal anti-σ(F) antagonist like domain. The crystal structures of two PAS domain constructs (at 2.3 and 1.6 Å) and a phosphatase/kinase dual domain construct (at 2.6 Å) are described. The PAS domain is shown to bind palmitic acid but to have 100 times greater affinity for palmitoleic acid. The full-length protein can exist in solution as both monomer and dimer. We speculate that a switch between monomer and dimer, possibly resulting from fatty acid binding, affects the accessibility of the serine of the C-terminal, anti-σ(F) antagonist domain for dephosphorylation by the phosphatase domain thus indirectly altering the availability of σ(F).
KW  - Bacterial Proteins: chemistry
KW  - Binding Sites
KW  - Catalytic Domain
KW  - Crystallography, X-Ray
KW  - Enzyme Assays
KW  - Fatty Acids: metabolism
KW  - Humans
KW  - Kinetics
KW  - Mycobacterium tuberculosis: enzymology
KW  - Phosphotransferases: chemistry
KW  - Protein Binding
KW  - Protein Multimerization
KW  - Protein Structure, Tertiary
KW  - Protein-Serine-Threonine Kinases: chemistry
KW  - Scattering, Small Angle
KW  - Structural Homology, Protein
KW  - X-Ray Diffraction
KW  - Bacterial Proteins (NLM Chemicals)
KW  - Fatty Acids (NLM Chemicals)
KW  - Phosphotransferases (NLM Chemicals)
KW  - PAS domain kinases (NLM Chemicals)
KW  - Protein-Serine-Threonine Kinases (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:21220116
UR  - <Go to ISI:>//WOS:000286348000009
DO  - DOI:10.1016/j.str.2010.11.010
UR  - https://bib-pubdb1.desy.de/record/144029
ER  -