Home > Publications database > Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment > print

001     141215
005     20250730162637.0
024 7 _ |a pmid:22516657
|2 pmid
024 7 _ |a 10.1016/j.abb.2012.04.003
|2 doi
024 7 _ |a 1096-0384
|2 ISSN
024 7 _ |a 0003-9861
|2 ISSN
024 7 _ |a WOS:000304789000004
|2 WOS
024 7 _ |a altmetric:112739834
|2 altmetric
024 7 _ |a openalex:W1980573223
|2 openalex
037 _ _ |a PHPPUBDB-24943
041 _ _ |a eng
082 _ _ |a 500
100 1 _ |a Paoletti, F.
110 1 _ |a DESY
|b European Molecular Biology Laboratory
245 _ _ |a Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment
260 _ _ |a San Diego, Calif.
|b Elsevier
|c 2012
300 _ _ |a 26-36
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a article
|2 DRIVER
336 7 _ |a Journal Article
|m journal
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
440 _ 0 |a Arch. Biochem. Biophys.
|v 522
|y 1
|x 0003-9861
|0 PERI:(DE-600)1461378-5
500 _ _ |3 Converted on 2013-05-30 09:54
500 _ _ |3 Converted on 2013-06-21 19:21
520 _ _ |a proNGF, the precursor of the neurotrophin NGF, is widely expressed in central and peripheral nervous system. Its physiological functions are still largely unknown, although it emerged from studies in the last decade that proNGF has additional and distinct functions with respect to NGF, besides acting chaperone-like for NGF folding during its biogenesis. The regulation of proNGF/NGF ratio represents a crucial process for homeostasis of brain and other tissues, and understanding the molecular aspects of these differences is important. We report the selection and characterization of a recombinant monoclonal anti-proNGF antibody in single chain Fv fragment (scFv) format. The selection exploited the Intracellular Antibody Capture Technology (IACT), starting from a naïve mouse SPLINT (Single Pot Library of INTracellular antibodies) library. This antibody (scFv FPro10) was expressed recombinantly in Escherichia coli, was proven to be highly soluble and stable, and thoroughly characterized from the biochemical-biophysical point of view. scFv FPro10 displays high affinity and specificity for proNGF, showing no cross-reactivity with other pro-neurotrophins. A structural model was obtained by SAXS. scFv FPro10 represents a new tool to be exploited for the selective immunoanalysis of proNGF, both in vitro and in vivo, and might help in understanding the molecular function of proNGF in neurodegeneration.
536 _ _ |0 G:(DE-H253)POF2-D1.2-20130405
|f POF II
|x 0
|c POF2-54G13
|a DORIS Beamline D1.2 (POF2-54G13)
588 _ _ |a Dataset connected to Pubmed
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Antibodies, Monoclonal: immunology
650 _ 2 |2 MeSH
|a Antibodies, Monoclonal: metabolism
650 _ 2 |2 MeSH
|a Antibody Affinity
650 _ 2 |2 MeSH
|a Base Sequence
650 _ 2 |2 MeSH
|a Circular Dichroism
650 _ 2 |2 MeSH
|a Cross Reactions
650 _ 2 |2 MeSH
|a DNA Primers
650 _ 2 |2 MeSH
|a Enzyme-Linked Immunosorbent Assay
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Isoelectric Focusing
650 _ 2 |2 MeSH
|a Mice
650 _ 2 |2 MeSH
|a Nerve Growth Factor: immunology
650 _ 2 |2 MeSH
|a Protein Precursors: immunology
650 _ 2 |2 MeSH
|a Recombinant Proteins: metabolism
650 _ 2 |2 MeSH
|a Scattering, Small Angle
650 _ 2 |2 MeSH
|a Single-Chain Antibodies: immunology
650 _ 2 |2 MeSH
|a Single-Chain Antibodies: metabolism
650 _ 2 |2 MeSH
|a Spectrometry, Fluorescence
650 _ 2 |2 MeSH
|a Surface Plasmon Resonance
650 _ 2 |2 MeSH
|a X-Ray Diffraction
650 _ 7 |0 0
|2 NLM Chemicals
|a Antibodies, Monoclonal
650 _ 7 |0 0
|2 NLM Chemicals
|a DNA Primers
650 _ 7 |0 0
|2 NLM Chemicals
|a Protein Precursors
650 _ 7 |0 0
|2 NLM Chemicals
|a Recombinant Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Single-Chain Antibodies
650 _ 7 |0 0
|2 NLM Chemicals
|a pro-nerve growth factor, mouse
650 _ 7 |0 9061-61-4
|2 NLM Chemicals
|a Nerve Growth Factor
693 _ _ |a DORIS III
|f DORIS Beamline D1.2
|1 EXP:(DE-H253)DORISIII-20150101
|0 EXP:(DE-H253)D-D1.2-20150101
|6 EXP:(DE-H253)D-D1.2-20150101
|x 0
700 1 _ |a Malerba, F.
700 1 _ |a Konarev, P. V.
700 1 _ |a Visintin, M.
700 1 _ |a Scardigli, R.
700 1 _ |a Fasulo, L.
700 1 _ |a Lamba, D.
700 1 _ |a Svergun, D. I.
700 1 _ |a Cattaneo, A.
773 _ _ |0 PERI:(DE-600)1461378-5
|a 10.1016/j.abb.2012.04.003
|g Vol. 522, p. 26-36
|p 26-36
|q 522<26-36
|t Archives of biochemistry and biophysics
|v 522
|x 0003-9861
|y 2012
856 4 0 |u http://www.sciencedirect.com/science/article/pii/S0003986112001348
909 C O |o oai:bib-pubdb1.desy.de:141215
|p VDB
910 1 _ |0 I:(DE-HGF)0
|a Externes Institut
|k Extern
913 1 _ |0 G:(DE-HGF)POF2-54G13
|1 G:(DE-HGF)POF2-540
|2 G:(DE-HGF)POF2-500
|9 G:(DE-H253)POF2-D1.2-20130405
|b Struktur der Materie
|v DORIS III
|x 0
|a DE-H253
|4 G:(DE-HGF)POF
|3 G:(DE-HGF)POF2
|l Forschung mit Photonen, Neutronen, Ionen
914 1 _ |y 2012
915 _ _ |a JCR/ISI refereed
|0 StatID:(DE-HGF)0010
915 _ _ |a Medline
|0 StatID:(DE-HGF)0300
|2 StatID
915 _ _ |a No Author Disambiguation
|0 StatID:(DE-HGF)1
|2 StatID
920 _ 1 |k EMBL
|i European Molecular Biology Laboratory
920 1 _ |0 I:(DE-H253)EMBL_-2012_-20130307
|k EMBL
|l European Molecular Biology Laboratory
|x 0
920 _ _ |k 001
980 _ _ |a PHPPUBDB
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a journal
980 _ _ |a I:(DE-H253)EMBL_-2012_-20130307
980 _ _ |a ConvertedRecord

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21