TY  - JOUR
AU  - Watson, A. A.
AU  - Mahajan, P.
AU  - Mertens, H. D.
AU  - Deery, M. J.
AU  - Zhang, W.
AU  - Pham, P.
AU  - Du, X.
AU  - Bartke, T.
AU  - Edlich, C.
AU  - Berridge, G.
AU  - Chen, Y.
AU  - Burgess-Brown, N. A.
AU  - Kouzarides, T.
AU  - Wiechens, N.
AU  - Owen-Hughes, T.
AU  - Svergun, D. I.
AU  - Gileadi, O.
AU  - Laue, E. D.
AU  - DESY
TI  - The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4
JO  - Journal of molecular biology
VL  - 422
SN  - 0022-2836
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PHPPUBDB-24948
SP  - 3-17
PY  - 2012
AB  - The NuRD (nucleosome remodeling and deacetylase) complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The core nucleosome remodeling function of the mammalian NuRD complex is executed by the helicase-domain-containing ATPase CHD4 (Mi-2β) subunit, which also contains N-terminal plant homeodomain (PHD) and chromo domains. The mode of regulation of chromatin remodeling by CHD4 is not well understood, nor is the role of its PHD and chromo domains. Here, we use small-angle X-ray scattering, nucleosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spectrometry to propose a three-dimensional structural model describing the overall shape and domain interactions of CHD4 and discuss the relevance of these for regulating the remodeling of chromatin by the NuRD complex.
KW  - Adenosine Triphosphatases: metabolism
KW  - Autoantigens: chemistry
KW  - Autoantigens: metabolism
KW  - Binding Sites
KW  - Chromatin: metabolism
KW  - Chromatin Assembly and Disassembly
KW  - Electrophoretic Mobility Shift Assay
KW  - Humans
KW  - Mi-2 Nucleosome Remodeling and Deacetylase Complex: chemistry
KW  - Mi-2 Nucleosome Remodeling and Deacetylase Complex: metabolism
KW  - Models, Biological
KW  - Nucleosomes: metabolism
KW  - Protein Structure, Tertiary
KW  - Proteolysis
KW  - Autoantigens (NLM Chemicals)
KW  - CHD4 protein, human (NLM Chemicals)
KW  - Chromatin (NLM Chemicals)
KW  - Nucleosomes (NLM Chemicals)
KW  - Mi-2 Nucleosome Remodeling and Deacetylase Complex (NLM Chemicals)
KW  - Adenosine Triphosphatases (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:22575888
C2  - pmc:PMC3437443
UR  - <Go to ISI:>//WOS:000308267700002
DO  - DOI:10.1016/j.jmb.2012.04.031
UR  - https://bib-pubdb1.desy.de/record/140870
ER  -