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@ARTICLE{Neues:140148,
author = {Neues, F. and Hild, S. and Epple, M. and Marti, O. and
Ziegler, A. and DESY},
title = {{A}morphous and crystalline calcium carbonate distribution
in the tergite cuticle of moulting {P}orcellio scaber
({I}sopoda, {C}rustacea)},
journal = {Journal of structural biology},
volume = {175},
issn = {1047-8477},
address = {San Diego, Calif.},
publisher = {Elsevier},
reportid = {PHPPUBDB-24461},
pages = {10-20},
year = {2011},
abstract = {The main mineral components of the isopod cuticle consists
of crystalline magnesium calcite and amorphous calcium
carbonate. During moulting isopods moult first the posterior
and then the anterior half of the body. In terrestrial
species calcium carbonate is subject to resorption, storage
and recycling in order to retain significant fractions of
the mineral during the moulting cycle. We used synchrotron
X-ray powder diffraction, elemental analysis and Raman
spectroscopy to quantify the ACC/calcite ratio, the mineral
phase distribution and the composition within the anterior
and posterior tergite cuticle during eight different stages
of the moulting cycle of Porcellio scaber. The results show
that most of the amorphous calcium carbonate (ACC) is
resorbed from the cuticle, whereas calcite remains in the
old cuticle and is shed during moulting. During premoult
resorption of ACC from the posterior cuticle is accompanied
by an increase within the anterior tergites, and
mineralization of the new posterior cuticle by resorption of
mineral from the anterior cuticle. This suggests that one
reason for using ACC in cuticle mineralization is to
facilitate resorption and recycling of cuticular calcium
carbonate. Furthermore we show that ACC precedes the
formation of calcite in distal layers of the tergite
cuticle.},
keywords = {Animal Structures: chemistry / Animal Structures:
metabolism / Animal Structures: ultrastructure / Animals /
Calcification, Physiologic / Calcium Carbonate: chemistry /
Calcium Carbonate: metabolism / Isopoda: chemistry /
Isopoda: metabolism / Isopoda: ultrastructure / Microscopy,
Electron, Scanning / Molting / Powder Diffraction / Spectrum
Analysis, Raman / X-Ray Diffraction / Calcium Carbonate (NLM
Chemicals)},
cin = {EMBL},
ddc = {540},
cid = {$I:(DE-H253)EMBL_-2012_-20130307$},
pnm = {DORIS Beamline B2 (POF2-54G13)},
pid = {G:(DE-H253)POF2-B2-20130405},
experiment = {EXP:(DE-H253)D-B2-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21458575},
UT = {WOS:000291371800002},
doi = {10.1016/j.jsb.2011.03.019},
url = {https://bib-pubdb1.desy.de/record/140148},
}