TY  - JOUR
AU  - Neues, F.
AU  - Hild, S.
AU  - Epple, M.
AU  - Marti, O.
AU  - Ziegler, A.
AU  - DESY
TI  - Amorphous and crystalline calcium carbonate distribution in the tergite cuticle of moulting Porcellio scaber (Isopoda,  Crustacea)
JO  - Journal of structural biology
VL  - 175
SN  - 1047-8477
CY  - San Diego, Calif.
PB  - Elsevier
M1  - PHPPUBDB-24461
SP  - 10-20
PY  - 2011
AB  - The main mineral components of the isopod cuticle consists of crystalline magnesium calcite and amorphous calcium carbonate. During moulting isopods moult first the posterior and then the anterior half of the body. In terrestrial species calcium carbonate is subject to resorption, storage and recycling in order to retain significant fractions of the mineral during the moulting cycle. We used synchrotron X-ray powder diffraction, elemental analysis and Raman spectroscopy to quantify the ACC/calcite ratio, the mineral phase distribution and the composition within the anterior and posterior tergite cuticle during eight different stages of the moulting cycle of Porcellio scaber. The results show that most of the amorphous calcium carbonate (ACC) is resorbed from the cuticle, whereas calcite remains in the old cuticle and is shed during moulting. During premoult resorption of ACC from the posterior cuticle is accompanied by an increase within the anterior tergites, and mineralization of the new posterior cuticle by resorption of mineral from the anterior cuticle. This suggests that one reason for using ACC in cuticle mineralization is to facilitate resorption and recycling of cuticular calcium carbonate. Furthermore we show that ACC precedes the formation of calcite in distal layers of the tergite cuticle.
KW  - Animal Structures: chemistry
KW  - Animal Structures: metabolism
KW  - Animal Structures: ultrastructure
KW  - Animals
KW  - Calcification, Physiologic
KW  - Calcium Carbonate: chemistry
KW  - Calcium Carbonate: metabolism
KW  - Isopoda: chemistry
KW  - Isopoda: metabolism
KW  - Isopoda: ultrastructure
KW  - Microscopy, Electron, Scanning
KW  - Molting
KW  - Powder Diffraction
KW  - Spectrum Analysis, Raman
KW  - X-Ray Diffraction
KW  - Calcium Carbonate (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:21458575
UR  - <Go to ISI:>//WOS:000291371800002
DO  - DOI:10.1016/j.jsb.2011.03.019
UR  - https://bib-pubdb1.desy.de/record/140148
ER  -