%0 Journal Article
%A Baranauskiene, L.
%A Hilvo, M.
%A Matuliene, J.
%A Golovenko, D.
%A Manakova, E.
%A Dudutiene, V.
%A Michailoviene, V.
%A Torresan, J.
%A Jachno, J.
%A Parkkila, S.
%A Maresca, A.
%A Supuran, C.
%A Grazulis, S.
%A Matulis, D.
%A DESY
%T Inhibition and binding studies of carbonic anhydrase isozymes I, II and IX with benzimidazo[1,2-c][1,2,3]thiadiazole-7-sulphonamides
%J Journal of enzyme inhibition and medicinal chemistry
%V 25
%@ 1475-6374
%C London
%I Informa Healthcare
%M PHPPUBDB-24676
%P 863-870
%D 2010
%X The binding and inhibition strength of a series of benzimidazo[1,2-c][1,2,3]thiadiazole-7-sulphonamides were determined for recombinant human carbonic anhydrase isoforms I, II, and IX. The inhibition strength was determined by a stop-flow method to measure carbon dioxide hydration. Inhibitor-enzyme binding was determined by two biophysical techniques-isothermal titration calorimetry and thermal shift assay. The co-crystal structure was determined by X-ray crystallography. Comparing the results obtained using three different inhibition and binding methods increased the accuracy of compound affinity ranking and the ability to determine compound inhibitory specificity towards a particular carbonic anhydrase isoform. In most cases, all three methods yielded the same results despite using very different approaches to measure the binding and inhibition reactions. Some of the compounds studied are submicromolar inhibitors of the isoform IX, a prominent cancer target.
%K Algorithms
%K Antigens, Neoplasm: genetics
%K Antigens, Neoplasm: metabolism
%K Antineoplastic Agents: chemistry
%K Antineoplastic Agents: metabolism
%K Antineoplastic Agents: pharmacology
%K Benzimidazoles: chemistry
%K Benzimidazoles: metabolism
%K Benzimidazoles: pharmacology
%K Calorimetry: methods
%K Carbonic Anhydrase I: antagonists & inhibitors
%K Carbonic Anhydrase I: genetics
%K Carbonic Anhydrase I: metabolism
%K Carbonic Anhydrase II: antagonists & inhibitors
%K Carbonic Anhydrase II: chemistry
%K Carbonic Anhydrase II: genetics
%K Carbonic Anhydrase II: metabolism
%K Carbonic Anhydrase Inhibitors: chemistry
%K Carbonic Anhydrase Inhibitors: metabolism
%K Carbonic Anhydrase Inhibitors: pharmacology
%K Carbonic Anhydrases: genetics
%K Carbonic Anhydrases: metabolism
%K Catalytic Domain: drug effects
%K Crystallography, X-Ray
%K Humans
%K Kinetics
%K Ligands
%K Molecular Conformation
%K Recombinant Proteins: antagonists & inhibitors
%K Recombinant Proteins: chemistry
%K Recombinant Proteins: metabolism
%K Sulfonamides: chemistry
%K Sulfonamides: metabolism
%K Sulfonamides: pharmacology
%K Thiadiazoles: chemistry
%K Thiadiazoles: metabolism
%K Thiadiazoles: pharmacology
%K Antigens, Neoplasm (NLM Chemicals)
%K Antineoplastic Agents (NLM Chemicals)
%K Benzimidazoles (NLM Chemicals)
%K Carbonic Anhydrase Inhibitors (NLM Chemicals)
%K Ligands (NLM Chemicals)
%K Recombinant Proteins (NLM Chemicals)
%K Sulfonamides (NLM Chemicals)
%K Thiadiazoles (NLM Chemicals)
%K Carbonic Anhydrase I (NLM Chemicals)
%K Carbonic Anhydrase II (NLM Chemicals)
%K CA9 protein, human (NLM Chemicals)
%K Carbonic Anhydrases (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:20166809
%U <Go to ISI:>//WOS:000283939300016
%R 10.3109/14756360903571685
%U https://bib-pubdb1.desy.de/record/140090