000558824 001__ 558824
000558824 005__ 20240927190602.0
000558824 0247_ $$aG:(GEPRIS)165173437$$d165173437
000558824 035__ $$aG:(GEPRIS)165173437
000558824 040__ $$aGEPRIS$$chttp://gepris.its.kfa-juelich.de
000558824 150__ $$aVisualizing the Role of Myeloid Cells During CNS Autoimmunity$$y2010 - 2016
000558824 371__ $$aProfessor Dr. Alexander Flügel
000558824 450__ $$aDFG project G:(GEPRIS)165173437$$wd$$y2010 - 2016
000558824 5101_ $$0I:(DE-588b)2007744-0$$aDeutsche Forschungsgemeinschaft$$bDFG
000558824 550__ $$0G:(GEPRIS)140392386$$aFOR 1336: Von Monozyten bis zu Hirnmakrophagen -Einflüsse auf die Eigenschaften myeloider Zellen im Gehirn$$wt
000558824 680__ $$aIn this project we will study the function of myeloid cells during the initiation of experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. The following questions will be followed: 1. What are the migratory steps of myeloid cells into the CNS during incipient EAE? 2. What are the molecular cues that mediate their transmigration of the blood brain barrier (BBB)? 3. What role do myeloid cells play in opening the BBB? 4. Which myeloid cells mediate the initial antigen presentation to autoaggressive encephalitogenic T cells? The methodic focus of this project lies in the combination of 2-photon laser scanning microscopy with functional/molecular analyses. Myeloid cells will be made visible by staining them with fluorescence dyes or by use of genetically encoded fluorescence proteins. The migratory behavior of the cells at the BBB and in the different CNS milieus and EAE phases will be visualized by live 2-PM. The analyses of the interactions of myeloid cells with autoagressive T cells in acute and chronic EAE models will be performed using functional markers which will be introduced into the T cells. These markers will make possible the visualization of the binding between T cells and myeloid APCs and the consecutive activation of the effector T cells.
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000558824 909CO $$ooai:juser.fz-juelich.de:984667
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000558824 980__ $$aAUTHORITY