TY  - JOUR
AU  - Hunter, Mark S.
AU  - Segelke, Brent
AU  - Messerschmidt, Marc
AU  - Williams, Garth J.
AU  - Zatsepin, Nadia A.
AU  - Barty, Anton
AU  - Benner, W. Henry
AU  - Carlson, David B.
AU  - Coleman, Matthew
AU  - Graf, Alexander
AU  - Hau-Riege, Stefan P.
AU  - Pardini, Tommaso
AU  - Seibert, M. Marvin
AU  - Evans, James
AU  - Boutet, Sébastien
AU  - Frank, Matthias
TI  - Fixed-Target Protein Serial Microcrystallography with an X-ray Free Electron Laser
JO  - Scientific reports
VL  - 4
IS  - 6026
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - PUBDB-2015-01262
SP  - 1
PY  - 2014
N1  - OA
AB  - We present results from experiments at the Linac Coherent Light Source (LCLS) demonstrating that serial femtosecond crystallography (SFX) can be performed to high resolution ( 2.5 Å) using protein microcrystals deposited on an ultra-thin silicon nitride membrane and embedded in a preservation medium at room temperature. Data can be acquired at a high acquisition rate using x-ray free electron laser sources to overcome radiation damage, while sample consumption is dramatically reduced compared to flowing jet methods. We achieved a peak data acquisition rate of 10 Hz with a hit rate of  38
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000340674000004
C6  - pmid:25113598
DO  - DOI:10.1038/srep06026
UR  - https://bib-pubdb1.desy.de/record/207279
ER  -