Journal Article

PHPPUBDB-24461

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Amorphous and crystalline calcium carbonate distribution in the tergite cuticle of moulting Porcellio scaber (Isopoda, Crustacea)

Neues, F. ; Hild, S. ; Epple, M. ; Marti, O. ; Ziegler, A. ; DESY

2011
Elsevier San Diego, Calif.

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Please use a persistent id in citations: doi:10.1016/j.jsb.2011.03.019

Abstract: The main mineral components of the isopod cuticle consists of crystalline magnesium calcite and amorphous calcium carbonate. During moulting isopods moult first the posterior and then the anterior half of the body. In terrestrial species calcium carbonate is subject to resorption, storage and recycling in order to retain significant fractions of the mineral during the moulting cycle. We used synchrotron X-ray powder diffraction, elemental analysis and Raman spectroscopy to quantify the ACC/calcite ratio, the mineral phase distribution and the composition within the anterior and posterior tergite cuticle during eight different stages of the moulting cycle of Porcellio scaber. The results show that most of the amorphous calcium carbonate (ACC) is resorbed from the cuticle, whereas calcite remains in the old cuticle and is shed during moulting. During premoult resorption of ACC from the posterior cuticle is accompanied by an increase within the anterior tergites, and mineralization of the new posterior cuticle by resorption of mineral from the anterior cuticle. This suggests that one reason for using ACC in cuticle mineralization is to facilitate resorption and recycling of cuticular calcium carbonate. Furthermore we show that ACC precedes the formation of calcite in distal layers of the tergite cuticle.

Keyword(s): Animal Structures: chemistry (MeSH) ; Animal Structures: metabolism (MeSH) ; Animal Structures: ultrastructure (MeSH) ; Animals (MeSH) ; Calcification, Physiologic (MeSH) ; Calcium Carbonate: chemistry (MeSH) ; Calcium Carbonate: metabolism (MeSH) ; Isopoda: chemistry (MeSH) ; Isopoda: metabolism (MeSH) ; Isopoda: ultrastructure (MeSH) ; Microscopy, Electron, Scanning (MeSH) ; Molting (MeSH) ; Powder Diffraction (MeSH) ; Spectrum Analysis, Raman (MeSH) ; X-Ray Diffraction (MeSH) ; Calcium Carbonate

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Contributing Institute(s):

1. European Molecular Biology Laboratory (EMBL)

Research Program(s):

1. DORIS Beamline B2 (POF2-54G13) (POF2-54G13)

Experiment(s):

1. DORIS Beamline B2 (DORIS III)

Appears in the scientific report 2011
Notes: (c) Elsevier Inc. Post referee full text in progress. Code P.

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Database coverage:
; ; JCR ; No Author Disambiguation ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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