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2017-06-23
13:59
[PUBDB-2017-06002] Journal Article
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Thermoresponsive behavior of poly(N-isopropylacrylamide)s with dodecyl and carboxyl terminal groups in aqueous solution: pH-dependent cloud point temperature
ddc:540
It was recently reported that poly(N-isopropyl acrylamide) (PNIPAm) polymers synthesized by RAFT polymerization using S-1-dodecyl-S′-(α,α′-dimethyl-α′′-acetic acid)trithiocarbonate as a chain transfer agent form micelles in aqueous solutions with the core of hydrophobic terminal dodecyl groups and the corona of PNIPAm chains with carboxylic groups at the periphery, the ionization of which prevents the micelles from phase separation above the lower critical solution temperature of PNIPAm in water (Langmuir 30:7986–7992). In this paper, we study the pH- and ionic strength-dependence of the aggregation behavior of two HOOC-PNIPAm-C12 polymers, differing in the degree of polymerization, in aqueous solutions. [...]
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2017-06-23
13:37
[PUBDB-2017-05998] Journal Article
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Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function
ddc:570
Phosducin (Pdc) is a conserved phosphoprotein that, when unphosphorylated, binds with high affinity to the complex of βγ-subunits of G protein transducin (Gtβγ). The ability of Pdc to bind to Gtβγ is inhibited through its phosphorylation at S54 and S73 within the N-terminal domain (Pdc-ND) followed by association with the scaffolding protein 14-3-3. [...]

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2017-06-23
13:36
OpenAccess [PUBDB-2017-05996] Journal Article
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Crystallographic and SAXS studies of S -adenosyl- L -homocysteine hydrolase from Bradyrhizobium elkanii
IUCrJ 4(3), 271 - 282 (2017) [10.1107/S2052252517002433]  GO
ddc:530
S-Adenosyl-L-homocysteine hydrolase (SAHase) from the symbiotic bacterium Bradyrhizobium elkanii (BeSAHase) was crystallized in four ligand complexes with (i) mixed adenosine (Ado) and cordycepin (Cord; 3′-deoxyadenosine), (ii) adenine (Ade), (iii) Ado and (iv) mixed 2′-deoxyadenosine (2′-dAdo) and Ade. The crystal structures were solved at resolutions of 1.84, 1.95, 1.95 and 1.54 Å, respectively. [...]
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2017-06-23
13:21
[PUBDB-2017-05995] Journal Article
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SAXS and other spectroscopic analysis of 12S cruciferin isolated from the seeds of Brassica nigra
ddc:610
Oilseeds of the plant family Brassicaceae are important for providing both lipid and protein contents to human nutrition. Cruciferins (12S globulins) are seed storage proteins, which are getting attention due to their allergenic and pathogenicity related nature. [...]
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2017-06-23
12:01
[PUBDB-2017-05974] Journal Article
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Significance of Individual Residues at the Regulatory Site of Yeast Pyruvate Decarboxylase for Allosteric Substrate Activation
Biochemistry 56(9), 1285 - 1298 (2017) [10.1021/acs.biochem.6b01158]  GO
ddc:570
The catalytic activity of the allosteric enzyme pyruvate decarboxylase from yeast is strictly controlled by its own substrate pyruvate via covalent binding at a separate regulatory site. Kinetic studies, chemical modifications, cross-linking, small-angle X-ray scattering, and crystal structure analyses have led to a detailed understanding of the substrate activation mechanism at an atomic level with C221 as the core moiety of the regulatory site. [...]

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2017-06-23
10:29
[PUBDB-2017-05931] Journal Article
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Formation of high thermally stable isoporous integral asymmetric block copolymer membranes
ddc:670
Tailor-made poly(α-methylstyrene)- and poly(4-methylstyrene)-block-poly(4-vinylpyridine) diblock copolymers with poly(4-vinylpyridine) as the minority component were synthesised via living anionic polymerisation. In bulk they show spherical or cylindrical microphases. [...]
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2017-06-23
09:51
[PUBDB-2017-05918] Journal Article
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Dicationic Surfactants with Glycine Counter Ions for Oligonucleotide Transportation
ChemPhysChem 17(15), 2424 - 2433 (2016) [10.1002/cphc.201600175]  GO
ddc:540
Gemini surfactants are good candidates to bind, protect, and deliver nucleic acids. Herein, the concept of amino acids (namely glycine) as counter ions of gemini surfactants for gene therapy application was explored. [...]
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2017-06-23
09:21
[PUBDB-2017-05909] Journal Article
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Molecular Precision and Enzymatic Degradation: From Readily to Undegradable Polymeric Micelles by Minor Structural Changes
ddc:540
Studying the enzymatic degradation of synthetic polymers is crucial for the design of suitable materials for biomedical applications ranging from advanced drug delivery systems to tissue engineering. One of the key parameters that governs enzymatic activity is the limited accessibility of the enzyme to its substrates that may be collapsed inside hydrophobic domains. [...]
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2017-06-22
16:24
OpenAccess [PUBDB-2017-05889] Journal Article
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Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin ?6?4
ddc:570
Integrin $\alpha6^{\beta}$4 is a major component of hemidesmosomes that mediate the stable anchorage of epithelial cells to the underlying basement membrane. Integrin $\alpha6^{\beta}$4 has also been implicated in cell proliferation and migration and in carcinoma progression. [...]
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2017-06-22
16:15
OpenAccess [PUBDB-2017-05885] Journal Article
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Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
ddc:000
Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. [...]
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