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OpenAccess [PUBDB-2017-06548] Journal Article
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Rapid cadmium SAD phasing at the standard wavelength (1Å)
Cadmium ions can be effectively used to promote crystal growth and for experimental phasing. Here, the use of cadmium ions as a suitable anomalous scatterer at the standard wavelength of 1 Å is demonstrated. [...]
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[PUBDB-2017-05445] Journal Article
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Human Δ 3 ,Δ 2 -enoyl-CoA isomerase, type 2: a structural enzymology study on the catalytic role of its ACBP domain and helix-10
The FEBS journal 282(4), 746 - 768 () [10.1111/febs.13179]  GO
The catalytic domain of the trimeric human Δ3,Δ2-enoyl-CoA isomerase, type 2 (HsECI2), has the typical crotonase fold. In the active site of this fold two main chain NH groups form an oxyanion hole for binding the thioester oxygen of the 3E- or 3Z-enoyl-CoA substrate molecules. [...]
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[PUBDB-2017-02967] Journal Article
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Structure of Toxoplasma gondii coronin, an actin-binding protein that relocalizes to the posterior pole of invasive parasites and contributes to invasion and egress
The FASEB journal 28(11), 4729 - 4747 () [10.1096/fj.14-252569]  GO
Coronins are involved in the regulation of actin dynamics in a multifaceted way, participating in cell migration and vesicular trafficking. Apicomplexan parasites, which exhibit an actin-dependent gliding motility that is essential for traversal through tissues, as well as invasion of and egress from host cells, express only a single coronin, whereas higher eukaryotes possess several isoforms. [...]
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Embargoed OpenAccess [PUBDB-2017-00403] Journal Article
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Structural and functional dissection of Toxoplasma gondii armadillo repeats only protein
Journal of cell science 129(5), 1031 - 1045 () [10.1242/jcs.177386]  GO
Rhoptries are club-shaped, regulated secretory organelles that cluster at the apical pole of apicomplexan parasites. Their discharge is essential for invasion and the establishment of an intracellular lifestyle. [...]
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[PUBDB-2016-02792] Journal Article
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Irradiation-induced degradation of PTB7 investigated by valence band and S 2 p photoelectron spectroscopy
Monochromatic radiation with known absolute radiant power from an undulator at the electron storage ring Metrology Light Source (MLS) was used to irradiate PTB7 (a thieno[3, 4-b]thiophene-alt-benzodithiophene polymer) thin films at wavelengths (photon energies) of 185 nm (6.70 eV), 220 nm (5.64 eV), 300 nm (4.13 eV), 320 nm (3.88 eV), 356 nm (3.48 eV) and 675 nm (1.84 eV) under ultra-high vacuum conditions for the investigation of radiation-induced degradation effects. The characterization of the thin films is focused at ultraviolet photoelectron spectroscopy (UPS) of valence bands and is complemented by S 2p x-ray photoelectron spectroscopy (S 2p XPS) before and after the irradiation procedure. [...]
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OpenAccess [DESY-2014-02662] Journal Article
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Structural Differences Explain Diverse Functions of Plasmodium Actins
PLoS pathogens 10(4), e1004091 () [10.1371/journal.ppat.1004091]  GO
Actins are highly conserved proteins and key players in central processes in all eukaryotic cells. The two actins of the malaria parasite are among the most divergent eukaryotic actins and also differ from each other more than isoforms in any other species. [...]
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