CFEL-XOM

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2018-08-22
14:26
OpenAccess [PUBDB-2018-03102] Journal Article
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Megahertz serial crystallography
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. [...]
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ddc:500
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2018-05-31
16:00
OpenAccess [PUBDB-2018-02164] Journal Article
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Enzyme intermediates captured “on the fly” by mix-and-inject serial crystallography
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ddc:570
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2018-02-02
16:01
OpenAccess [PUBDB-2018-01024] Journal Article
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Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals
High-resolution Bragg diffraction from aerosolized single granulovirus nano-crystals using an X-ray free-electron laser is demonstrated. The outerdimensions of the in-vacuum aerosol injector components are identical toconventional liquid-microjet nozzles used in serial diffraction experiments,which allows the injector to be utilized with standard mountings. [...]
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ddc:540
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2017-10-24
11:53
OpenAccess [PUBDB-2017-11523] Journal Article
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Analysis of XFEL serial diffraction data from individual crystalline fibrils
IUCrJ 4(6), 795 - 811 () [10.1107/S2052252517014324]  GO
Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections [...]
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ddc:530
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2017-10-05
15:34
[PUBDB-2017-11182] Journal Article
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Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers
The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are often accompanied by significant background scattering. [...]
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ddc:540
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2017-03-16
15:22
OpenAccess [PUBDB-2017-01394] Journal Article
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Double-flow focused liquid injector for efficient serial femtosecond crystallography
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. [...]
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ddc:000
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2016-12-19
13:19
OpenAccess [PUBDB-2016-06339] Journal Article
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Extreme ultraviolet resonant inelastic X-ray scattering (RIXS) at a seeded free-electron laser
In the past few years, we have been witnessing an increased interest for studying materials properties under non-equilibrium conditions. Several well established spectroscopies for experiments in the energy domain have been successfully adapted to the time domain with sub-picosecond time resolution. [...]
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ddc:000
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2016-12-16
10:33
OpenAccess [PUBDB-2016-06266] Journal Article
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Structural enzymology using X-ray free electron lasers
Structural dynamics 4(4), 044003 () [10.1063/1.4972069]  GO
Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. [...]
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ddc:530
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2016-12-13
08:58
OpenAccess [PUBDB-2016-06119] Journal Article
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Jet delivery system for Raman scattering on bio-inorganic compounds
Applied physics letters 109(21), 213502 () [10.1063/1.4967476]  GO
We present a micro-jet sample delivery system for Raman measurements. Compared to cuvette measurements, the observed Raman signal is enhanced by more than one order of magnitude and does not contain signal distortions from the liquid-glass interface. [...]
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ddc:530
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2016-11-30
10:41
OpenAccess [PUBDB-2016-05742] Journal Article
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Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. [...]
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ddc:610
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