CFEL-I

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2017-10-20
12:14
OpenAccess [PUBDB-2017-11466] Preprint/Report
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Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals
[arXiv:1702.04014] ddc:530
We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray-scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. [...]
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2017-10-10
16:07
[PUBDB-2017-11258] Conference Presentation
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Laser-induced acoustic desorption of large molecules via tape-drive method
DPG-Frühjahrstagung SAMOP, Meeting locationHannover, Germany, 29 Feb 2016 - 4 Mar 20162016-02-292016-03-04

By dispersing molecular beams with strong electric fields, we can routinely produce pure samples of individual conformers or clusters according to their quantum state. Selected molecules can be further controlled using alignment and orientation by laser and static electric fields [1]. [...]
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2017-09-14
21:02
[PUBDB-2017-10788] Journal Article
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Structure determination based on continuous diffraction from macromolecular crystals
ddc:570
Bright and coherent X-ray sources, such free-electron lasers, have spurred large activities in developing new methods to obtain the structures of biological macromolecules. In particular, single-molecule diffraction is highly desired, as it would abolish the need for crystallization. [...]
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2017-09-14
10:07
DBCoverage [PUBDB-2017-10745] Journal Article
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Mix-and-diffuse serial synchrotron crystallographyMix-and-diffuse serial synchrotron crystallography
ddc:530
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2017-09-08
07:44
[PUBDB-2017-10518] Journal Article
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Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization
ddc:540
A new approach is described to screen for protein nanocrystals based on the reversibility of crystallization. Methods to characterize nanocrystals are in strong need to facilitate sample preparation for serial femtosecond X-ray nanocrystallography (SFX). [...]
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2017-08-31
16:31
OpenAccess [PUBDB-2017-10204] Journal Article
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Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
ddc:610
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. [...]
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2017-08-11
22:16
[PUBDB-2017-09193] Contribution to a book
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Opportunities for Structure Determination Using X-ray Free-electron Laser Pulses
X-Ray Free Electron Lasers / Bergmann, Uwe (Editor); Cambridge : Royal Society of Chemistry, 2017 doi:10.1039/9781782624097
X-Ray Free Electron Lasers / Bergmann, Uwe (Editor); Cambridge : Royal Society of Chemistry, 2017 doi:10.1039/9781782624097
Cambridge : Royal Society of Chemistry, Energy and Environment Series, 397 - 417 (2017) [10.1039/9781782624097-00397]  GO
One of the exciting prospects enabled by the short, intense pulses of X-ray free-electron lasers (XFELs) is the determination of near-atomic resolution structures of biomolecules and their complexes without the need of crystallisation or cryo-cooling. Using femtosecond pulses that freeze all atomic motion could open up entirely new capabilities and insights into the dynamics, function and interaction of many systems in structural biology. [...]
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2017-08-01
15:10
OpenAccess [PUBDB-2017-08794] Journal Article
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Incoherent Diffractive Imaging via Intensity Correlations of Hard X Rays
ddc:550
Established x-ray diffraction methods allow for high-resolution structure determination of crystals, crystallized protein structures, or even single molecules. While these techniques rely on coherent scattering, incoherent processes like fluorescence emission—often the predominant scattering mechanism—are generally considered detrimental for imaging applications [...]
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2017-07-25
10:57
DBCoverage [PUBDB-2017-08413] Journal Article
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Single-shot determination of focused FEL wave fields using iterative phase retrieval

Optics express 25(15), 17892 - 17903 (2017) [10.1364/OE.25.017892]  GO
ddc:530
Determining fluctuations in focus properties is essential for many experiments at Self-Amplified-Spontaneous-Emission (SASE) based Free-Electron-Lasers (FELs), in particular for imaging single non-crystalline biological particles. We report on a diffractive imaging technique to fully characterize highly focused, single-shot pulses using an iterative phase retrieval algorithm, and benchmark it against an existing Hartmann wavefront sensor. [...]
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2017-07-10
21:35
OpenAccess [PUBDB-2017-07304] Journal Article
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FELIX: an algorithm for indexing multiple crystallites in X-ray free-electron laser snapshot diffraction images
ddc:540
A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented. The algorithm, FELIX, utilizes a generalized parametrization of the Rodrigues–Frank space, in which all crystal systems can be represented without singularities [...]
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